You are hired at a laboratory and are asked to design a PCR (RT-PCR) assay to rapidly test people for COVID19 infection. You need to make sure that the original COVID19 strain and the two new variants (Ltmeb variant and Silly variant) can be detected and distinguished in a single PCR assay. The protocol for the assay is already established, you just have to identify the right primers to identify all three strains shown below. COVID19 is a single-stranded RNA virus, however, your colleague took the original and the two new strains and generated large amounts of double-stranded cDNA for you to develop the detection assay.
1) Classify variants based on the kind of mutations they have acquired, based on the changes they cause to the DNA? Justify Answer Original strain 5'atg tct ctg gga cca atg gta cta aga ggt ttg ata acc ctg tcc tac cat tta atg atg gtg ttt att ttg ctt cca ctg aga agt cta aca taa 3' 3'tac aga gat cct ggt tac cat gat tct cca aac tat tgg gac agg atg gta aat tac tac cac aaa taa aac gaa ggt gac tct tca gat tgt att 5' Ltmeb Variant 5'atg tct ctg gga cca atg gta eta aga ggt ttg ata ace etg tee tac eat tta atg atg gtg ttt att ttg ctt cca ctg aga agt cta aca taa 3' 3'tac aga gat cct ggt tac cat gat tet cea aac tat tgg gac agg atg gta aat tac tac cac aaa taa aac gaa ggt gac tct tca gat tgt att 5' Silly Variant 2 5'atg tct ctg gga cca atg gta cta aga ggt ttg ata acc ctg tcc tac tcc tac cat tta atg atg gtg ttt cat tta atg_atg_gtg_ttt att ttg ctt cca ctg aga agt cta aca taa 3' 3'tac aga gat cct ggt tac cat gat tct cca aac tat tgg gac agg atg gta aat tac tac cac aaa taa aac gta aat tac tac cac aaa taa aac gaa ggt gac tct tca gat tgt att 5'
The original COVID-19 strain and the two new strains (Ltmeb and Silly variant) need to be distinguished and detected in a single PCR assay. The PCR assay would use cDNA generated from the double-stranded DNA of the original COVID-19 and the two variants that your colleague created. A pair of primers are designed to bind to a complementary sequence in the cDNA and act as a starting point for the PCR reaction. These primers are essential to distinguish and detect the three strains. Variants classification based on the kind of mutations they have acquired, based on the changes they cause to the DNA:
The three variants of COVID-19 mentioned above - original strain, Ltmeb variant, and Silly variant differ from each other based on mutations in their DNA sequence. These mutations can be classified into three main categories: synonymous, non-synonymous, and frameshift.
Synonymous mutation: A mutation that does not change the amino acid encoded by a codon is called synonymous mutation. For example, the original COVID-19 strain has a codon 79 in which the original base adenine is replaced by guanine in the Ltmeb and Silly variants. This is a synonymous mutation, as the same amino acid methionine is encoded by both the original and mutated codons.
Non-synonymous mutation: When a mutation changes the amino acid encoded by a codon, it is called non-synonymous mutation. Both Ltmeb and Silly variants have a non-synonymous mutation at codon 265, where the original base thymine is replaced by cytosine and adenine, respectively. This results in the substitution of tyrosine by histidine in Ltmeb variant and asparagine in the Silly variant.
Frameshift mutation: When a nucleotide is added or deleted from the DNA sequence, it causes a frameshift mutation. Silly variant has a frameshift mutation at position 261-262 where a guanine is added causing a shift in the reading frame. The mutation introduces a premature stop codon, resulting in the production of a truncated protein. Thus, the Silly variant produces a shorter version of the spike protein than the original strain and Ltmeb variant. Hence, we need to identify the right set of primers that could detect all three strains based on the mutations they have acquired.
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Which is a short-term effect of m0lestation?
Answer: Some of the short-term impacts can include severe pain and/or the need to seek urgent medical help, pregnancy, sexually transmitted infections, and unusual bodily changes.
Explanation:
hope this helps.
help me somebody i'm desperate
Answer:
a decision
Explanation:
you chose not to cheat. it was your decision
Answer:
both a consequence and a decision
Explanation:
you choose not to because of the consequences if getting caught and feeling guilty
When comparing convenience diet food to organic foods, we can conclude __________.
A.
that organic food is more expensive
B.
that convenience food has more additives
C.
that organic food has less nutrition
D.
that convenience food is better for you
Answer:
B. that convenience food has more additives.
Explanation:
an example is a convenience store apple and a organic apple. and convenience food have at least to max of 0 to 12 common additives. anyways organic apples rot faster to prevent this convenient store apples have a thin layer of edible wax.
Answer:B
Explanation:
When Jackson gets stressed out over a big decision, he makes a pros and cons list for different courses of action. Which kind of strategy is he using to deal with his stress? O Downward comparison O Emotion-focused coping O Problem-focused coping
abnormal crackle-like lung sounds heard through a stethoscope during inspiration. true or false?
True : abnormal lung sounds during inspiration, audible with a stethoscope.
What is stethoscope ?
The stethoscope is a medical instrument used for auscultation, or hearing internal sounds in an animal or human body. The conventional configuration includes one or two tubes attached to two earpieces, a small disc-shaped resonator pressed up against the skin. In addition to hearing blood flow in arteries and veins, a stethoscope can be used to listen to sounds made by the heart, lungs, or intestines. When monitoring blood pressure, it is frequently used in conjunction with a manual sphygmomanometer.
Less frequently, "mechanic's stethoscopes," which have chest pieces in the shape of rods, are used to hear the internal noises made by machines, such as listening to an automobile engine to diagnose a malfunction by hearing the sounds of its internal parts.
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theories are designed to help guide the practice of health education.
Theories play a crucial role in health education as they provide a foundation for understanding how individuals think, learn, and behave. These theories serve as valuable tools for health education practitioners in designing effective programs, interventions, and strategies aimed at promoting behavior change.
Additionally, theories aid in comprehending the reasons behind risky behaviors and the factors influencing behavior modification. Several theories commonly used in health education include the Health Belief Model, Social Cognitive Theory, Transtheoretical Model, and Diffusion of Innovations Theory.
The Health Belief Model is utilized to predict and elucidate individuals' health behaviors. According to this model, people are more likely to modify their behavior if they perceive themselves to be susceptible to a health issue, perceive the problem as severe, perceive the benefits of change to outweigh the costs, and have confidence in their ability to enact the change.
The Social Cognitive Theory suggests that individuals learn by observing others. Health education practitioners can employ this theory to promote learning from role models and foster self-efficacy for behavior change. The Transtheoretical Model posits that individuals progress through distinct stages when making behavior changes. Health education practitioners can apply this model to tailor interventions and strategies to individuals at different stages of change.
Lastly, the Diffusion of Innovations Theory proposes that individuals learn about novel ideas, products, or services through opinion leaders and adopt them based on perceived usefulness and ease of use. Health education practitioners can utilize this theory to encourage the adoption of new health behaviors and practices.
In conclusion, theories are indispensable in health education as they provide a framework for understanding human behavior and guide the development of effective interventions. The Health Belief Model, Social Cognitive Theory, Transtheoretical Model, and Diffusion of Innovations Theory are among the theories commonly employed in health education, each offering valuable insights into behavior change processes and strategies.
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Given the relatively modest number of calories burned by anything but the most vigorous activities, why can people consume over 2,000 kilocalories a day, yet maintain a healthy body weight?
i. Not all calories are created equal, so a person who consumes 2,000 kilocalories of sugar- and fat-laden processed food will gain weight, whereas a person who consumes 2,000 calories of nonprocessed, low-carbohydrate food will not.
ii. People really should exercise vigorously for one to two hours per day.
iii. They can't, and this has led to a problem of obesity in the United States.
iv. Most of the energy consumed in food is spent maintaining the body's functions, so only a fraction of food energy needs to be burned in exercise.
Not all calories are created equal, meaning that the source and composition of the calories consumed can have a significant impact on weight gain or loss.
When someone consumes 2,000 kilocalories of sugar- and fat-laden processed food, the body may store excess energy as fat, leading to weight gain. On the other hand, consuming 2,000 calories of non processed, low-carbohydrate food typically provides a better balance of nutrients and can be more satiating, making it less likely to result in weight gain. The composition of the calories consumed, rather than the sheer number of calories, plays a crucial role in weight management. Highly processed foods with high sugar and fat content can lead to weight gain, while consuming whole, minimally processed foods can help maintain a healthy body weight. It's important to focus on the quality of calories consumed, including a balanced intake of nutrients, to support overall health and weight management.
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A single-case experimental design is similar to a(n)
Single-subject research is similar to group research—especially experimental group research—in many ways. They are both quantitative approaches that try to establish causal relationships by manipulating an independent variable, measuring a dependent variable, and controlling extraneous variables.
A single-case experimental design is similar to a case study.
Single-case experimental design is a research method used to study the behavior of an individual or a small group of individuals. It involves measuring a behavior multiple times before and after a specific intervention is introduced to see if the intervention caused a change in the behavior.
This design is similar to a case study in that it focuses on a specific individual or group and collects detailed data about their behavior. However, unlike a case study, a single-case experimental design involves manipulating an independent variable to see its effect on a dependent variable.
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What is health and human services 12 about?
Answer:
operating the divisions including 9 agencies in us public service
what makes a potential a factor in the job field
Answer:
If you enjoy your job and the pay is adequate, you can make a good living.
I hope this helps you
:)
Answer:
Here's some factors relating to workers ↓
Employee growth. This is very important to monitor to make sure your workers are at their full learning drive.How many people does the company have? This is also important because it can be good and bad.Worker skill tree. I recommend making one because it can help monitor your employees' growth and overall skill.Net worth. Your net worth is how much money you have made, or money you make on average.Success. This is very important because if your business is not successful, and you invested lots of money into that business, it will likely be a failure unless some miracle is pulled.
Write a story about a character who is addicted to drugs, goes through the addiction cycle, and then breaks the addiction cycle?
Which of the following situations describes the ideal way to lift a patient?
Answer: what are the answer options?
Explanation:
Do two lines that are not parallel always, sometimes, or never intersect? Explain.
never; When two lines are not parallel, they are in separate planes.
never; When two lines are not parallel, they are in separate planes.
never; You cannot draw two lines that are not parallel and do not intersect.
never; You cannot draw two lines that are not parallel and do not intersect.
sometimes; The two lines might be far enough apart in the plane to never intersect.
sometimes; The two lines might be far enough apart in the plane to never intersect.
sometimes; The two lines could be in parallel planes, but not parallel to each other.
sometimes; The two lines could be in parallel planes, but not parallel to each other.
always; Lines that are not parallel are lines that intersect.
Answer:
always
Explanation:
lines that are not parallel are lines that intersect.
Two lines which are not parallel always; the two lines which are not parallel are the lines that intersect each other. Thus, the correct option is E.
What are parallel lines?In geometry, the two parallel lines are coplanar infinite straight lines which do not intersect at any given point. Parallel planes are the planes in the same three-dimensional space which never meet each other. Parallel curves or lines are the curves which do not touch each other or which do not intersect and keep a fixed minimum distance from each other.
The parallel lines can be both in horizontal and vertical direction. We can see parallel lines which are the examples in our daily life like a zebra crossing, the lines of notebooks, and on the railway tracks around us.
Therefore, the correct option is E.
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as a youth to your community how will you share your knowledge to the other?
explain properly. i give brainliest asap
A nurse assess a patient with on set ms. which clinical manifestion would the nurse expect to find?
Answer: Nystagmus
Explanation: Early signs and symptoms of MS include changes in motor skills, vision, and sensation. Hyperresponsive reflexes, excessive somnolence, and heat intolerance
Individuals with anorexia nervosa concentrate on which body cue?
1.Hunger
2.Controlling food intake
3.Anxiety
4.Weakness
Answer:
2. Controlling food intake.
Explanation:
Individuals with anorexia nervosa concentrate on controlling food intake.
Hope this helps!
Individuals with anorexia nervosa concentrate heavily on controlling food intake and their weight, often to the point of extreme calorie restriction.
They may also focus on specific body cues such as perceived "flaws" or areas they want to make smaller. These cues can include their stomach, thighs, or hips. The individual may become fixated on these body parts and may engage in excessive exercise or restrictive eating patterns to achieve their desired body shape. However, hunger and weakness can also be a part of anorexia nervosa, as individuals may feel guilty or ashamed for eating and may push themselves to go without food for long periods of time. Ultimately, anorexia nervosa is a complex mental health disorder that involves a range of physical and psychological symptoms that require professional treatment and support.
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What equation relates to muscular strength?
Answer:
A simple prediction equation to estimate your 1RM is (0.033 x RTF x load) + load. In this equation, "RTF" is the number of repetitions you completed and "load" is the amount of weight you lifted.
Explanation:
I majored in Health
How can some types of asthma be prevented?
A. by increasing insulin levels
B. by avoiding mold
C. by avoiding high fat foods
D. by starting an exercise program
Answer:
I think is b.
Explanation:
I'm not sure tho but it's most likely
Common safety equipment carried on the ambulance includes all of the following, EXCEPT
a. face shields.
b. hazardous materials gear.
c. safety goggles.
d. turnout gear.
One item that would not typically be included in this list of safety equipment is turnout gear. (option d. turnout gear)
Turnout gear is typically worn by firefighters rather than ambulance personnel, and is designed to protect against heat and flames rather than biological hazards or other dangers that may be encountered during medical emergencies. The common safety equipment carried on an ambulance includes a variety of items that are essential for ensuring the safety of both patients and medical personnel.
These may include items such as gloves, masks, gowns, eye protection, and even hazardous materials gear. While it's important for ambulance crews to have access to a wide range of safety equipment, it's equally important to ensure that the gear they carry is appropriate for the types of risks they may face on the job. Answer is option d. turnout gear.
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the medical term for the hearing of sounds within the body through a stethoscope is:
The medical term for the hearing of sounds within the body through a stethoscope is: Auscultation.
Auscultation is used to aid in the diagnosis of many medical conditions, and to listen for physiological abnormalities. To perform auscultation, the doctor will place the stethoscope on the patient's body, often on the chest, back, or abdomen. The doctor then listens for sounds, such as heart and lung sounds, as well as any abnormal sounds.
Abnormal sounds can indicate the presence of disease and could include abnormal heart murmurs, breath sounds, and the presence of fluid in the lungs. Auscultation is a key component of the physical examination and can be used to assess a variety of conditions. It is a noninvasive method of diagnosing medical conditions that can provide valuable information for the doctor.
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Shards of metal are examples of what type of contamination
Answer:
Physical contamination occurs when physical objects find their way into foods. These could include dirt, hair, bandages, metal shavings, glass, jewelry, etc. Natural objects, such as fish bones, are also considered physical contaminants.
Explanation:
Hope this helps :)
Shards of metal are examples of physical contamination.
Pollution is a term to refer to the situation in which a new element or substance enters (in high concentrations) a specific environment that alters the balance of the ecosystem. Pollution has several types such as:
Physical contamination: Produced when an object is physically breaking into an ecosystem in which it should not be naturally.Chemical contamination: Produced when a chemical reaction occurs that modifies the compounds producing harmful reactions.Biological contamination: Produced by the presence of biological organisms that can affect normality in any ecosystem.According to the above, metal fragments are an example of physical contamination because they are fragments of a material that is not normally found on the surface of the earth, but below it as a mineral.
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The nurse is assisting at the scene of a mass casualty. how would the nurse triage each victim?
How do nurses screen each victim?
Patients fall into the following treatment priority categories during triage at mass casualty events:
Triage card:
Triage cards are color-coded by category. Victims are given a triage card in that color after being evaluated, making it easy to identify those who need immediate medical attention. There are other cards that can be used for this, but the most commonly used at UK mass casualty events is the SMART tag card folded in the manner shown below. These provide a quick notes area and a visual representation of patient triage categories. - Accidents are considered imminent when urgent medical attention and life-saving measures are required.
BLACK: (Deceased/expectant) injuries not compatible with life or not capable of causing spontaneous respiration; should not be moved to the collecting place
RED: Taken to the collecting location first; (immediate) severe injuries, but high chance of survival with treatment
YELLOW: Serious injuries but not yet life-threatening (delayed).
GREEN: Minor injuries (Walking Wounded)
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Why is it that teens that are more physically active need a significantly different dietary
plan than teens who are much less active?
Physically active teens need a significantly different diet plan because teenage years are a time of rapid physical and mental development, which increases the need for calories and the majority of nutrients.
When the teenagers are physically active, they need more energy in the form of calories for their activities. Due to their physical activeness, the nutrients which they intake gets depleted easily, hence they require more protein and carbohydrates in their diet for overall growth and muscle build up as compared to the teens with lesser physical activities.
What are Dietary plans? A diet that preserves or enhances general health is considered healthy. The body receives the key nutrients it needs from a balanced diet, including hydration, macronutrients like protein, micronutrients like vitamins, and sufficient amounts of fiber and food energy. Fruits, vegetables, and whole grains can be found in a healthy diet, along with little to no processed food and unnaturally sweetened beverages. The nutrients needed for a balanced diet can be obtained from both plant-based and animal-based foods, while vegans must obtain their vitamin B12 from a non-plant source. Governmental and medical organizations offer a variety of nutrition recommendations to inform people about the right foods to eat for health.In some nations, it is also required to include nutrition facts on food packaging so that consumers can compare the nutritional value of various items.To learn more about Dietary plans, refer to:
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What is the Main idea of abortions
Answer: Medical procedure that ends a pregnancy.
Which of the following should pregnant woman avoid
Answer: there is no screenshot or anything of answer choices but pregnant women should avoid alcohol, hair dye, don't do too much caffeine, don't breathe smoke and don't smoke yourself. Also don't eat certain foods
Explanation:
IS this a good score!!????
Answer:
yes, is a good score :) congratulations
Explanation:
Which is the best example of muscular endurance ? Plato
Long-distance running is a sport that requires muscular endurance. During a race, a marathon runner's body performs the same movement and stride, over and over again. This requires their muscles to have an advanced level of endurance to avoid injury or extreme fatigue.
The ancient Egyptians began public health and sanitation systems. Choose the answer. True O False
healthcare acronyms
Pharagraph by words
Answer:
there are many of them
Explanation:
-- Assistive Care Services
ACT Assertive Community Treatment
ACTION Advocates Committed to Improving Our Nursing
ACTS ASPEN Compaints/Incidents Tracking System
ACYF` HHS Administration for Children, Youth and Families
AD -- Advance Directive
-- Alzheimer's Disease
ADA -- Aged/Disabled Adult waiver
-- American Dental Association
-- Americans with Disabilities Act
ADAPT Alzheimer's Disease Anti-Inflammatory Prevention Trial
ADC -- AAHSA Development Corporation
-- Adult Day Care
ADD -- Administration on Developmental Disabilities
-- Attention Deficit Disorder
Add Health National Longitudinal Study of Adolescent Health
ADEA Age Discrimination in Employment Act
ADH Arkansas Department of Health
ADHD Attention Deficit Hyperactivity Disorder
ADI Alzheimer's Disease Initiative
ADL Activity of Daily Living
ADR Adverse Drug Reaction
ADRD Alzheimer's Disease or a Related Disorder
ADS Automated Discharge Summary
ADSA Aging and Disability Services Administration
ADT Admission, Discharge, Transfer
AE Adaptive Environment
AEM ASPEN Enforcement Module
AFC Adult Foster Care
AFCARS Adoption and Foster Care Analysis and Reporting System
AFCH -- Adult Family-Care Home
-- Adult Foster Care Home
AFDC Aid to Families with Dependent Children
AFH -- Adult Family Home
-- Adult Foster Home
AFMC Arkansas Foundation for Medical Care
AGH Adult Group Home
AGI Adjusted Gross Income
AHA American Hospital Association
AHC Academic Health Centers
AHCA -- Agency for Health Care Administration
-- American Health Care Association
AHCCCS Arizona Health Care Cost Containment System
AHCPR HHS Agency for Health Care Policy and Research (now the Agency for Healthcare Research and Quality)
AHEAD Asset and Health Dynamics Among the Oldest Old
AHEC Area Health Education Center
AHIC American Health Information Community
AHIMA American Health Information Management Association [Direct Link]
AHRQ HHS Agency for Healthcare Research and Quality (previously the Agency for Health Care Policy and Research)
AHS American Housing Survey
AIDS Acquired Immune Deficiency Syndrome
AIG American International Group
AIME Average Indexed Monthly Earning
AIP Annual Implementation Plan
AK Alaska
AL Alabama
AL+ Assisted Living Plus
AL/RC Assisted Living/Residential Care
ALC Assisted Living Community
ALE Assisted Living for the Elderly waiver
ALF Assisted Living Facility
ALFA Assisted Living Federation of America
ALP Assisted Living Program
ALR Assisted Living Residence
ALR-RC Assisted Living Residence-Residential Care
ALR-SRHC Assisted Living Residence-Supported Residential Health Care
ALS Assisted Living Score
ALSA Assisted Living Service Agency
ALTCS Arizona Long-Term Care Systems
ALU Assisted Living University
ALW Assisted Living Workgroup
ALWPP Assisted Living Waiver Pilot Project
AMA American Medical Association
AMCRA American Managed Care and Review Association
AMDA American Medical Directors Association
AMSO American Medical Specialty Organization [Direct Link]