A molecular technologist has run a vertical gel. While analyzing the gel, the technologist notices that the bands in the outer lanes did not run as far as similar-sized bands in the inner lanes, so the bands look like a smile on the gel. What is the explanation for this

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Answer 1

The phenomenon of bands appearing like a smile on a vertical gel, where the bands in the outer lanes do not run as far as similar-sized bands in the inner lanes, is known as smiling or concave distortion. This distortion can occur due to several factors:

Uneven electric field distribution: The electric field in the gel may not be distributed evenly across the entire gel, resulting in variations in the migration rate of DNA or other molecules. This uneven distribution could be caused by uneven buffer concentration or temperature gradients within the gel.

Heat generation: During electrophoresis, heat is generated due to the electric current passing through the gel. This heat can cause localized variations in the gel temperature, leading to variations in the gel matrix and affecting the migration of the molecules. In particular, heat buildup near the edges of the gel can slow down the migration of DNA bands, resulting in the smiling effect.

pH gradients: pH gradients can occur within the gel due to differences in buffer concentration or pH stability. These pH gradients can affect the charge density and mobility of the molecules being separated, leading to variations in their migration rates and causing the bands to smile.

Gel composition: Variations in the gel composition, such as uneven polymerization or differences in pore size, can lead to variations in the sieving properties of the gel. This can result in different migration rates for molecules in different regions of the gel, causing the bands to appear distorted.

To minimize or prevent smiling in vertical gels, it is important to ensure even buffer distribution, maintain consistent temperature throughout the gel, avoid excessive heat generation, and carefully prepare the gel to achieve uniform composition and pore size. Additionally, using proper gel-loading techniques and applying an even electric field across the gel can help reduce or eliminate this distortion.

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Related Questions

2. Explain brightness of light using the wave model of light.

Answers

Answer:

the wave model of light is useful for explaining brightness, color, and the frequency-dependent bending of light at a surface between media. For example, students could observe some of the wave behaviors or light by observing that when light passes through a small opening the waves spread out. They could observe that if the wavelength is short, the waves spread out very little, whereas longer wavelengths spread out more

Explanation:

write the ionic equation for dissolution and the solubility product (ksp) expression for each of the following slightly soluble ionic compounds: (a) pbcl2 (b) ag2s (c) sr3(po4)2 (d) srso4

Answers

The ionic equation for dissolution and the solubility product (ksp) expression for each of the following slightly soluble ionic compounds.

(a) For PbCl2:
Dissolution: PbCl2 (s) → Pb²⁺ (aq) + 2Cl⁻ (aq)
Ionic equation: PbCl2 (s) ⇌ Pb²⁺ (aq) + 2Cl⁻ (aq)
Solubility product (Ksp) expression: Ksp = [Pb²⁺][Cl⁻]²

(b) For Ag2S:
Dissolution: Ag2S (s) → 2Ag⁺ (aq) + S²⁻ (aq)
Ionic equation: Ag2S (s) ⇌ 2Ag⁺ (aq) + S²⁻ (aq)
Solubility product (Ksp) expression: Ksp = [Ag⁺]²[S²⁻]

(c) For Sr3(PO4)2:
Dissolution: Sr3(PO4)2 (s) → 3Sr²⁺ (aq) + 2PO₄³⁻ (aq)
Ionic equation: Sr3(PO4)2 (s) ⇌ 3Sr²⁺ (aq) + 2PO₄³⁻ (aq)
Solubility product (Ksp) expression: Ksp = [Sr²⁺]³[PO₄³⁻]²

(d) For SrSO4:
Dissolution: SrSO4 (s) → Sr²⁺ (aq) + SO₄²⁻ (aq)
Ionic equation: SrSO4 (s) ⇌ Sr²⁺ (aq) + SO₄²⁻ (aq)
Solubility product (Ksp) expression: Ksp = [Sr²⁺][SO₄²⁻]

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a scale model of the solar system

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There you go BRAINLIEST would be appreciated
a scale model of the solar system

Explain what happens in:a. RBC with 10% NaCl.b. RBC with 0.9% NaCl.c. RBC with 0.1% NaCl.

Answers

a. In RBC with 10% NaCl, the concentration of sodium chloride is much higher than the normal concentration in blood, causing the red blood cells to shrink and become crenated due to osmotic pressure.

b. In RBC with 0.9% NaCl, the concentration of sodium chloride is close to the normal concentration in blood, resulting in little to no change in the shape or size of the red blood cells.

c. In RBC with 0.1% NaCl, the concentration of sodium chloride is much lower than the normal concentration in blood, causing the red blood cells to swell and become distorted due to osmotic pressure.

What is the effect of 10% NaCl on RBC?

The effect of 10% NaCl on RBC is that it causes the water to move out of the RBC and into the solution, resulting in the RBC shrinking and becoming crenated due to the high concentration of salt compared to the concentration inside the RBC.

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Shorter product life cycles have led to increased demand uncertainty and difficulty in forecasting. Select one: D O True O False

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The given statement “Shorter product life cycles have led to increased demand uncertainty and difficulty in forecasting” is true because shorter product life cycles have led to increased demand uncertainty and difficulty in forecasting. It is becoming more difficult to predict demand, and there is a higher probability of product failure than there was in the past.

There are several factors responsible for this increased demand uncertainty and difficulty in forecasting. One of the most significant factors is the decrease in product life cycle length. Shorter product life cycles imply that new items and designs are being introduced on a more frequent basis.Product life cycles are the stages that a product passes through from conception to eventual obsolescence. It starts with the development of the product and continues until the product is no longer in use. It includes the introduction stage, growth stage, maturity stage, and decline stage.A product's life cycle has an impact on supply chain management since it has a significant impact on demand forecasting. As a result, any adjustments in demand forecasts must be accompanied by adjustments in supply chains. In a nutshell, shorter product life cycles have resulted in increased demand uncertainty and difficulty in forecasting, making it more challenging to manage supply chains effectively.

So, shorter product life cycles have led to increased demand uncertainty and difficulty in forecasting is true.

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Carbon is one of a few elements that bonds to itself. The scientific term for this is

Answers

Answer:

Alkanes

Explanation:

Explanation:

The answer is Alkanes.Alkanes are compound that consists entirely of atoms of carbon and hydrogen joined to one another by single bonds

which element has the following configuration: [xe]6s24f4 ? enter the chemical symbol for the element.

Answers

The element with the configuration [Xe]6s24f4 is Gadolinium (Gd).


In the given electron configuration, "[Xe]" represents the electron configuration of the noble gas xenon (54 electrons). The following part, "6s24f4," indicates the distribution of the remaining electrons in the outer shells. The "6s2" portion indicates that there are two electrons in the 6s orbital, and the "4f4" indicates that there are four electrons in the 4f orbital.
Gadolinium is a chemical element with the atomic number 64 and the symbol Gd. It belongs to the lanthanide series of elements and is part of the f-block in the periodic table. Gadolinium is a silvery-white metal that exhibits magnetic properties and has various applications, including its use in medical imaging, nuclear reactors, and electronic devices.

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6. Which of the following labels for the picture below is correct? a) Solute + solvent = solution b) Solvent + solute = saturation c) Solvent + solute = soluble d) Solvent + solute = solution ​

Answers

Answer:

answer is a since solute dissolve a solvent to give a solution

The law that relates the temperature and volume of a gas to each other is known as.

Answers

the asnwser will be helium

how to find the electron in an atom/element

Answers

Answer:

to find the number of electrons an element has locate it on the periodic table of elements find the atomic number and note the number of protons because they are naturally electrically neutral

Answer:

M-A=N

Explanation:

M-A=N

Here is an example.

The equation above means that the atomic number (A) subtracted from the average atomic mass (M) equals the combined amount of neutrons and protons. Since we know that 35 17Cl is Chlorine (this is because Chlorine (Cl) is the 17th number on the periodic table and has the average atomic mass of 35), we can insert our data into the equation and end up with the following:

35-17=18.

From here, we can tell that we have a mix of neutrons and protons, with the total being 18. Since the atomic number is 17, we can reasonably assume that there are 17 protons and 1 neutron.

But we still need to find the number of electrons. Fortunately, the number of electrons is always equivilant to the number of protons and the atomic mass, so we know that the number of electrons is 17.

So, we have;

17 Protons

1 Neutron

17 Electrons

In the quantum mechanical model, what two words do they use to describe
particles?

Answers

Answer:

This question is incomplete

Explanation:

This question is incomplete but one can assume the answer from the definition of quantum mechanics. Quantum mechanics is the science that describes particles in nature at the atomic and subatomic particle (electron, proton and neutron) level. Thus it can be assumed that atoms and subatomic particles are used to broadly describe particles.

Is this equation right? ​

Is this equation right?

Answers

Answer:

no

Explanation:

because the characters are invalid

Answer:

no

Explanation:

because they are not valid

Two water solutions that have a density of
1.00 g/mL are mixed, and a reaction occurs.
The temperature rises from 25.0°C to 27.0°C.
Given that the heat capacity is 4.18 J/g x°C
and that the total mass of the water is 75.0 g,
what is the heat change for the reaction?

Two water solutions that have a density of1.00 g/mL are mixed, and a reaction occurs.The temperature

Answers

Answer : 627 JBrainliest

The heat change for the reaction is 627 J.

What is heat change?

Heat change is the change in the heat content of a substance as a result of temperature change.

The heat change = mass × specific heat capacity × temperature change

Heat change = 75 × 4.18 × (27 - 25)

Hear change = 627 J

Therefore, the heat change for the reaction is 627 J.

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What was the carbon cycle on the prairie like?

Answers

Answer:

Explanation:

Carbon cycle explains the movement of carbon between the earth's biosphere, geosphere, hydrosphere and atmosphere. ... Carbon atoms are then released as carbon dioxide when organisms respire. The formation of fossil fuels and sedimentary rocks contribute to the carbon cycle for very long periods.

please help me please

please help me please

Answers

I think it is A,C,D hope this helps:)

God Bless

If you make an electromagnet using a battery circuit and a steel nail, how can you increase its magnetic strength?


Use a longer nail.


Wrap more coils of wire around the nail.


Replace the nail with a copper rod.


Remove the plastic insulation from the wire coil.
I would like a little help with this please

Answers

Answer:

B. Wrap more coils of wire around the nail

Increasing the number of coils in an electromagnet increases the strength of the magnetic field.

Explanation:

Why do scientists say that every investigation is not an experiment?

Answers

Answer:

Students will know that scientists find answers to questions about the natural world in different ways. ... Students will be able to explain that some science investigations are not experiments because they DON'T involve testing a hypothesis by changing one variable while keeping the other factors constant.

Explanation:

It  really all depends on the levels of suspicion.

represent three different ligands. which choices show a pair of geometric isomers (diastereomers) that have the same connection among atoms, but a different spatial orientation that cannot be described as a mirror image?

Answers

The three different ligands can be any type of compound or molecule with the ability to donate a pair of electrons to a metal ion. The pair of geometric isomers with the same connection among atoms, but different spatial orientation, can be determined by looking at the ligands' orientation around the metal ion.

Ligands are molecules or ions that bond to a central metal ion through a coordinate covalent bond, meaning they donate a pair of electrons to the metal ion. Ligands can have different structures and can be classified according to their coordination number, geometry, and donor atoms.

When a metal ion is surrounded by a set of ligands, the orientation of the ligands around the metal ion can lead to the formation of different stereoisomers.

Geometric isomers, also known as diastereomers, are a type of stereoisomers that have the same connectivity of atoms but a different spatial orientation that cannot be described as a mirror image.

To determine the pair of geometric isomers with the same connection among atoms but different spatial orientation, one needs to examine the ligands' orientation around the metal ion.

One example of geometric isomers could be found in a square planar coordination complex with two bidentate ligands. The two ligands could be oriented in a cis or trans configuration, resulting in two geometric isomers with the same connectivity of atoms but different spatial orientation.

Another example could be a coordination complex with two different monodentate ligands. The two ligands could be oriented in a facial or meridional configuration, leading to two geometric isomers with the same connection among atoms but a different spatial orientation.

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I'LL GIVE BRAINLIEST TO WHO ANSWERS FULL QUESTION FIRST
Example 3: Dry ice (CO2) sublimes at -78°C. What is this temperature in K?​

I'LL GIVE BRAINLIEST TO WHO ANSWERS FULL QUESTION FIRSTExample 3: Dry ice (CO2) sublimes at -78C. What

Answers

Answer:

Any solid may be sublimed if its temperature and pressure are below its triple point. For example, if you were to freeze water into ice and cool it further and then reduce the pressure, you could get ice to sublime. Some other examples include: Dry ice (solid CO2) which sublimes at atmospheric pressure and -78 C, and C60 (buckyballs) which sublimes at atmospheric pressure at around 800 K.

Explanation:

5.08 moles of an ideal gas are contained in a 3000-mL container at a temperature of 25 °C. What pressure does the gas exert, in mm Hg, on the container?

Answers

Answer:

eeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeiiiiiieeeeeemmmmmmmmiemeeeeeiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiiimiiiimmmmmmmmmmmmmmmmmmmiimmiimiiiiiiiimiiiiiimmii

Explanation:

A radioactive substance decreases by 65% each hour. Find the hourly decay factor. The hourly decay factor is__

Answers

A radioactive substance decreases by 65% each hour. Find the hourly decay factor. The hourly decay factor is 0.35.

Chemicals in the class of radionuclides (also known as radioactive materials) have unstable atomic nuclei. They become stable by undergoing modifications in the nucleus (spontaneous fission, alpha particle emission, neutron conversion to protons, or the opposite).

A radioactive atom will naturally emit radiation in the form of energy or particles in order to transition into a more stable state. The difference between radioactive material and the radiation it emits must be made.

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what four fundamental assumptions about atoms and matter make up modern atomic theory?

Answers

Modern atomic theory is based on four fundamental assumptions about atoms and matter. The first assumption is that all matter is composed of atoms, which are the smallest units of matter that can exist independently.

The second assumption is that atoms of the same element are identical in size, mass, and other properties, but atoms of different elements have different properties. The third assumption is that atoms combine in simple whole number ratios to form compounds. Finally, the fourth assumption is that chemical reactions involve the rearrangement of atoms, but the atoms themselves are not created or destroyed.

These four assumptions have been developed through experiments and observations over time, and they form the basis of modern atomic theory. Modern atomic theory is based on four fundamental assumptions about atoms and matter. First, all matter is composed of tiny, indivisible particles called atoms. Second, atoms of the same element have identical properties, while atoms of different elements have distinct properties.

Third, atoms combine in specific proportions to form compounds, following the law of definite proportions. Lastly, in chemical reactions, atoms are rearranged, conserved, and never created nor destroyed, adhering to the law of conservation of mass. These assumptions serve as the foundation for understanding atomic structure and chemical behavior.

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4) How many grams are in 4.63 x 102 moles of CC14?

Answers

Answer:

n=mass/molar mass

mass=?,molar mass=12+(35.5)4=154g/mol.n=4.63x10²

mass=4.63x10²x154=7128g

calculate the concentration of an aqueous solution of ca(oh)2 that has a ph of 11.31.

Answers

The concentration of the aqueous solution of Ca(OH)2 with a pH of 11.31 is approximately 0.00126 M.

To calculate the concentration of Ca(OH)2, we first determine the pOH of the solution by subtracting the pH from 14: pOH = 14 - 11.31 = 2.69. The pOH represents the negative logarithm (base 10) of the hydroxide ion concentration.

Using the pOH value, we can find the concentration of OH- ions: [OH-] = 10^(-pOH) = 10^(-2.69) ≈ 0.00126 M.

Since each formula unit of Ca(OH)2 dissociates into two OH- ions, the concentration of Ca(OH)2 is twice the concentration of OH- ions. Therefore, the concentration of the Ca(OH)2 solution is approximately 2 * 0.00126 M, which is 0.00252 M.

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hydrogen cyanide (hcn) is a poisonous gas that can be produced in the lab by reacting propane (c 3h 8) with nh 3 to produce hcn and h 2 (all in the gaseous state). how many grams of ammonia are required to produce 14.4 g of hcn if the reaction runs to 65% completion? lets solve this question in a series of steps: when properly balanced the coefficients for each species in the reaction are:

Answers

If the reaction proceeds to 65% completion, 13.95 grammes of ammonia are needed to make 14.4 grammes of HCN.

The balanced chemical equation for the reaction between propane and ammonia to produce hydrogen cyanide and hydrogen gas is:

C₃H₈ + NH₃ → HCN + 3H₂

According to the equation, one mole of NH₃ produces one mole of HCN. Therefore, to determine the number of moles of NH₃ required to produce 14.4 g of HCN, we need to convert the given mass of HCN into moles:

14.4 g HCN x (1 mole HCN/27.03 g HCN) = 0.5331 moles HCN

Since the reaction runs to 65% completion, the actual number of moles of HCN produced will be:

0.5331 moles HCN x 100/65 = 0.8202 moles HCN

Therefore, the number of moles of NH₃ required to produce this amount of HCN will be the same:

0.8202 moles NH₃

To convert moles of NH₃ to grams, we can use the molar mass of NH₃:

0.8202 moles NH3 x 17.03 g NH₃/mole = 13.95 g NH₃

Therefore, approximately 13.95 grams of ammonia are required to produce 14.4 g of HCN if the reaction runs to 65% completion.

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According to Einstein's equation, if 1 kg of mass "disappears" during a
reaction, then———-


A. the law of conservation of momentum is also violated.
OB. the law of conservation of charge is also violated.
C. the law of conservation of mass is violated.
OD. 3 x 108 J of energy is produced.

Answers

The answer is c

Because you can’t create/destroy energy it just doesn’t disappear. It changes from one form to another form of energy.

Uranium has three isotopes with the following percent abundances: 234- (0.0058%), 235- (0.71%), 238- (99.23%). Without doing any calculations, what do you expect the atomic mass of uranium to be in whole numbers. Why?

Answers

Answer:

Because it is the same element but has different atomic mass

anne added a sample of hydrochloric acid to water and determined the pH to be 1.79. what is the poH of the solution?

Answers

Answer: 12.21

Explanation:

It is well known that the sum of pH + pOH = 14.

To get the pOH of the solution, we subtract 1.79 from 14.

pH + pOH = 14

pOH = 14 - 1.79 = 12.21

pOH = 12.21

What occurs in an exothermic reaction?

A. A smell is produced
B. There is an increase in temperature.
C. Fizzing happens.
D. There is a color change.
Reset Selection

Answers

Answer:

B

Explanation:

exo - thermic     exo  as  external    thermo like thermometer

  external heat....heat is produced and given off

the answer would be d i think

Medicine. A pharmaceutical company conducts an experiment in which a subject takes 100mg of a substance orally. The researchers measure how many minutes it takes for half of the substance to exit the bloodstream. What kind of variable is the company studying?

Answers

The researchers may use statistical analysis to estimate the half-life and evaluate the substance's pharmacokinetic properties, such as absorption, distribution, metabolism, and elimination.

The variable that the pharmaceutical company is studying in this experiment is a pharmacokinetic variable known as the "half-life" of the substance. The half-life represents the time it takes for the concentration or amount of a substance in the bloodstream to decrease by half.

In this case, the researchers are administering 100mg of the substance orally to the subject and then measuring the time it takes for half of the substance to be eliminated from the bloodstream. The half-life is a crucial parameter in pharmacokinetics as it provides information about the rate of elimination or clearance of the substance from the body.

The half-life variable is a quantitative variable because it represents a measurable quantity, specifically the time duration. It is a continuous variable as it can take any positive value on the time scale, depending on the specific substance being studied. The researchers may use statistical analysis to estimate the half-life and evaluate the substance's pharmacokinetic properties, such as absorption, distribution, metabolism, and elimination.

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