typical, well aerated culture will have a density of about 109 or 1010 cfu/mL after an overnight incubation. If you are working with a strain of bacteria grows to 2x1010 cfu/mL after overnight incubation, how should you dilute and plate that culture to get 30-300 colonies on your plate
We have that the dilution factor to get 30-300 colonies is mathematically given as
dilution factor=10^8
Dilution factor
Question Parameters:
A density of about 109 or 1010 cfu/mL.
plate that culture to get 30-300 colonies on your plate.
Generally, Concentration of cells in the culture
C= 2x10^10 cfu/mL
Let's goal 200 colonies.
Let's extent plated be 1 mL
\(C= \frac{(number of colonies \times dilution factor)}{volume plated }\)
2e10 = 200\times dilution
dilution factor= \(\frac{2x10^10}{200}\)
dilution factor=10^8
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Complete Question
A typical, well-aerated culture will have a density of about 10^9 or 10^10 cfu/mL after an overnight incubation. If you are working with a strain of bacteria that grows to 2x10^10 cfu/mL after overnight incubation, how should you dilute and plate that culture to get 30-300 colonies on your plate? What dilution should you use?
Can you guys please help me
i'm no scientist
i hope this can help least i can do
"Generally, electromagnetic radiation is classified by wavelength into radio wave, microwave, terahertz (or sub-millimeter) radiation, infrared, the visible region we perceive as light, ultraviolet, X-rays, and gamma rays. The behavior of electromagnetic radiation depends on its wavelength."
7.Name the stages of photosynthesis and explain them in brief.
Aubrey uses the following formula to calculate a required value. Which elements of the formula use mixed cell referencing?
Answer:
TBH $1
Explanation:
Answer:
$1A
Explanation:
PLATO
Explain how cell division and cell multiplication follow a pre-
designed program stored in the DNA and what may happen
when cells become uncontrolled and anarchic.
• What is the possible relationship between Psalm 139:13-16
and the pre-design “instructions” that cells follow during
cell division and multiplication?
Cell division follows a pre-designed program due to differential gene expression, whereas uncontrolled division leads to cancer.
What is cell differentiation?Cell differentiation is the gene expression process that leads to the development of specialized cell types.
Cancer cells are specific cells that divide in an uncontrolled manner and thus they can generate tumors.
In conclusion, cell division follows a pre-designed program due to differential gene expression, whereas uncontrolled division leads to cancer.
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Help me pls what characteristics of life does a pregnant lady have and her baby
Which term describes a type of trait that is usually expresses only when an organism has two identical alleles for the trait?
A trait that is usually expressed only when an organism has two identical alleles for the trait is called a "recessive trait." When an organism has two different alleles for a trait, the trait that is expressed is called a "dominant trait."
Select the correct answer. Which of these periods contain elements with electrons in s, p, d, and f orbitals? A. periods 1–3 B. periods 1–4 C. periods 3–5 D. periods 6–7
In the periodic table, the periods represent the horizontal rows. Each period corresponds to a different principal energy level, and the elements within that period have electrons occupying specific orbitals.The correct answer is D. periods 6-7.
In periods 1-3, the elements only have electrons occupying the s and p orbitals. The d orbitals are not filled in these periods. In periods 1-4, the elements still only have electrons occupying the s and p orbitals. The d orbitals are again not filled in these periods.
In periods 3-5, the elements continue to have electrons in the s and p orbitals. However, in period 4, the d orbitals start to fill up, specifically in the transition metals. So, this option is not correct.
In periods 6-7, the elements have electrons in the s, p, d, and f orbitals. The f orbitals are filled in the lanthanide and actinide series, which are found in periods 6 and 7 respectively. Therefore, this option is correct. Thus, the correct answer is D. periods 6-7.
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why blood is classified as tissue in cellular organization?
Answer:
Blood is considered as a connective tissue .
Connective tissues have cells scattered throughout an extracellular matrix. They connect different body systems. Blood is considered as a type of connective tissue because of two reasons.
(i) Like the other connective tissues, blood is mesodermal in origin.
(ii) It connects the body systems, transports oxygen and nutrients to all the parts of the body, and removes the waste products. Blood has an extra-cellular matrix called plasma, with red blood cells, white blood cells, and platelets floating in it.
Answer:
Connective tissues have cells scattered throughout an extracellular matrix. They connect different body systems. Blood is considered as a type of connective tissue because It connects the body systems, transports oxygen and nutrients to all the parts of the body, and removes the waste products
hope this helps you
parent one and parent 2, I NEED HELP PLEASEEEEEEEEE, I WILL GIVE BRAINLEST I NEED HELPP, URGENTTTTTTTTT,
Answer:
The correct answer would be :
rr (parent I) × rr (parent II)
Explanation:
In this given question there are two traits present in the corns that are red kernels and yellow kernels. It is also given that the yellow kernel is dominant over the yellow kernels.
In the provided Punnett square and on our knowledge we know that the recessive trait which is the yellow kernel only expresses itself when both the genes of the kernel have recessive gene r.
In the heterozygous cross, there would be only 50% chances of the kernels of an ear of corn to produce yellow kernels.. There is only one possible condition which is:
rr cross with rr
r r
r rr rr
r rr rr
All kernels in this condition will be yellow.
What is a beta particle? How is it released from the atom?
So, an atom consists of protons, electrons, and neutrons. The nucleus of the atom is only composed of protons and neutrons, and electrons are surrounding the atom like a circle pretty much. So, a beta particle is an electron that happened to be in the nucleus, as a result of an unstable neutron dissecting into a proton and an electron. Since electrons are not supposed to be in the nucleus of an atom, the electron, or the beta particle, is released from the nucleus, if not the atom itself. The reason this occurrence transpires is to prevent the death of the atom. I hope that this has helped answer your question for you. Enjoy your day, and take care.
What types of proteins are synthesized by bound ribosomes?
Bound ribosomes are responsible for the synthesis of membrane proteins and secretory proteins.
Bound ribosomes are ribosomes that are attached to the endoplasmic reticulum (ER) or nuclear envelope. They are responsible for the synthesis of proteins that are destined for secretion or for incorporation into membranes. Membrane proteins are typically synthesized by bound ribosomes that are associated with the rough ER. These proteins are then inserted directly into the ER membrane or transported to other organelles such as the Golgi apparatus for further processing and sorting.
Secretory proteins are also synthesized by bound ribosomes and are transported to the ER lumen where they are folded and processed before being transported to the Golgi apparatus for further modification and sorting.
Overall, bound ribosomes play a crucial role in the synthesis of proteins that are essential for the proper function and survival of the cell.
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What can result when winds blow across ocean water
n
During the dry season in the Sahara
Desert, many animals compete for
limited sources of water.
Explain how limited abiotic factors
can impact the biotic factors in an
ecosystem.
OUTPUT
STATION
could you explain what you have to do
assume that the kinetics of a phosphorylation reaction carried out by a kinase can be appropriately described by the michaelis-menten relationship. competitive inhibition of this kinase by a drug molecule will
Competitive inhibition of a kinase by a drug molecule will increase the Km value and have no effect on the Vmax value, while non-competitive inhibition will decrease the Vmax value and have no effect on the Km value.
Assuming that the kinetics of a phosphorylation reaction carried out by a kinase can be appropriately described by the Michaelis-Menten relationship, competitive inhibition of this kinase by a drug molecule will lead to an increase in the Michaelis-Menten constant (Km) and no change in the maximum reaction rate (Vmax).
Competitive inhibition occurs when a drug molecule binds to the active site of the enzyme, preventing the substrate from binding and therefore inhibiting the reaction. This leads to an increase in the Km value, as more substrate is required to achieve the same reaction rate. However, the Vmax remains unchanged, as the maximum reaction rate can still be achieved if enough substrate is present to outcompete the inhibitor.
In contrast, non-competitive inhibition occurs when a drug molecule binds to a site other than the active site, causing a conformational change in the enzyme and reducing its activity. This leads to a decrease in the Vmax value, as the maximum reaction rate is reduced, but no change in the Km value.
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What is extinction?
What are the causes of the extinction of animals?
How the extinction of animals It can be fixed?
Answer:
Extinction is the termination of a kind of organism or of a group of kinds (taxon), usually a species. ... A typical species becomes extinct within 10 million years of its first appearance, although some species, called living fossils, survive with little to no morphological change for hundreds of millions of years.
Explanation:
# CAUSES OF ANIMAL EXTINCTION
Demographic and genetic phenomena.
Destruction of wild habitats.
Introduction of invasive species.
Climate change.
Hunting and illegal trafficking.
# Basic causes of extinction are as follows
2. Excessive hunting and killing of wild animals.
3. Poaching for purpose of meat, skin, fur, ivory, and rhino horns etc.
4. Capture and export of new species in large number.
5. Introduction of exotic species.
6. Excessive use of pesticides, fungicides, and other toxic chemicals.
7. Conversion of fertile land in the desert by overgrazing of domestic animals.
8. Deforestation leading to loss of covers or shelter to wild animals.
9. Excessive use of plant wood for timber, charcoal or fire purpose.
What happens to pressure and temperature as we go from the Earth's crust to the Earth's core?
Answer:Temperature and pressure progressively increase with increased proximity to Earth’s core. Recent studies indicate the core’s temperature may be close to 11,000 degrees Fahrenheit; that’s nearly 2,000 degrees warmer than previously thought and hotter than the surface of the Sun, according to a 2013 Forbes article.
Explanation:
The enzyme salivary amylase will act on starch, but not on protein. This action illustrates that salivary amylase
a) contains starch
b) is not reusable
c) has a specific substrate
d) is actually a carbohydrate
Answer:
C
Explanation:
Enzymes are substrate specific hence salivary amylase can only work on its appropriate substrate (starch)
What characteristic was used in this mission to determine a banana's relationship to other the plants?
The characteristic used to determine a banana's relationship to other plants is its morphology or physical characteristics.
What is morphology?
Morphology is the study of the form and structure of living organisms, including their physical and anatomical features. In biology, morphology is used to describe the appearance, shape, and size of different parts of an organism, such as its organs, tissues, cells, and even its molecules. This includes the study of external and internal structures, as well as their function, development, and evolution. Morphological characteristics are often used in the classification and identification of different species, as they can provide important clues about an organism's evolutionary history, ecological niche, and relationship to other organisms.
We assume you are referring to the scientific classification of bananas in the plant kingdom. In this case, the characteristic used to determine a banana's relationship to other plants is its morphology or physical characteristics. Bananas belong to the genus Musa, which is part of the family Musaceae.
The characteristics used to classify plants into different taxonomic groups include their structural features, such as their leaves, stems, flowers, and fruit, as well as their genetic and evolutionary relationships. These characteristics are used to group plants into increasingly broader taxonomic categories, from species to genus, family, order, class, phylum, and kingdom.
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The theory of natural selection is based upon which of the following?
Group of answer choices
rock layers
fossil evidence and observed living organisms
distribution of organisms throughout the world
breeding organisms with favorable traits
Answer:
Breeding organisms with favorable traits.
Explanation:
Natural selection is all about how an animal's traits help them survive where they live.
characteristics of contaminated water
Contaminated water is a type of water that is harmful to human beings due to the presence of harmful substances, pollutants, or impurities that make the water unsafe for drinking or other household uses. The characteristics of contaminated water can be identified through various indicators, which can either be physical, biological or chemical.
Physical indicators are visible and may include color, taste, and odor, while biological indicators are not visible and may include bacteria, viruses, and protozoa. Chemical indicators may include heavy metals, pesticides, and organic compounds, among others.
Physical Characteristics: Physical characteristics of contaminated water include cloudy or turbid appearance, unusual taste or odor, or discoloration of the water. The color may range from yellow to brown, blue to green, or even black.
Biological Characteristics: Biological characteristics of contaminated water include the presence of bacteria, viruses, or protozoa. These microorganisms can cause diseases such as diarrhea, typhoid, cholera, and dysentery.
Chemical Characteristics: Chemical characteristics of contaminated water include the presence of heavy metals such as lead, arsenic, or cadmium. Pesticides and fertilizers can also contaminate water and affect human health. Organic compounds like benzene and toluene can also be present in contaminated water, which can lead to health problems such as cancer and nerve damage.
Therefore, it is important to test water sources regularly to identify and monitor any contaminants that may be present. This can help prevent health problems associated with the use of contaminated water.
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A person has the genotype of RR and her phenotype is that she is right-handed. What would be the phenotype of an individual with the genotype of Rr?Answers Both ( right& left)Right-handed Left handedNone of these I think it is right-handed
According to the text, the phenotype is given by two alleles: R and r.
R causes the individual to be right-handed, so r causes the individual to be left-handed.
The dominant alleles are symbolized with capital letters while the recessive alleles are symbolized with small letters. This means that when the R allele is present, the phenotype will be right-handed regardless of whether the r allele is present. Therefore, the phenotypes of the different genotypes would be as follows:
RR = right-handed
Rr = right-handed
rr = left-handed
In saltwater, the water that surrounds a fish has a higher concentration of dissolved salts than is found in the fish's body cells. Which two methods allow the fish to maintain water balance? A. Pumping out salt through the gills B. Absorbing water into the body by osmosis C. Releasing only a little water in urine D. Taking in salts via food
The two methods that allow fish to maintain water balance in saltwater are pumping out salt through the gills and absorbing water into the body by osmosis.
In saltwater, the concentration of dissolved salts is higher in the surrounding water than in the fish's body cells. To maintain water balance, fish have developed two main methods:
1. Pumping out salt through the gills: Fish possess specialized cells in their gills that actively pump out excess salts from their bodies. This process helps maintain a lower salt concentration within the fish's body, preventing dehydration and maintaining water balance.
2. Absorbing water into the body by osmosis: Osmosis is the movement of water from an area of lower solute concentration to an area of higher solute concentration through a semi-permeable membrane. Fish have a protective layer of skin that allows water to be absorbed into their bodies while preventing the entry of excessive salts.
By absorbing water through their skin, fish replenish the water lost to the surrounding saltwater, helping to maintain their internal water balance.
These two mechanisms work together to enable fish to survive in saltwater environments. By actively pumping out salts and absorbing water, fish can regulate their internal salt concentration and prevent dehydration, ensuring their overall water balance is maintained.
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FIRST RIGHT ANSWER GETS BRAINLYEST OR WHATEVER
Water molecules usually end up as:
a.
Carbon
c.
Nitrogen
b.
Water molecules
d.
Blood
Answer:
The answer would be C) Nitrogen
Explanation:
Answer:
I think is A
please mark me as brainliest
What is the MOST frequent cause of slash and burn deforestation?
A. office buildings
B. canals like the Panama Canal
C. National Parks
D. agriculture
2. The number of grizzly bear deaths in Alberta from 1976 to 1988 was estimated to be 581. Only 281 deaths were recorded from 1988 to 2000. How does this information affect the prediction you made in question 1? Explain your answer.
The data shows a significant decrease in grizzly bear deaths from 581 in the earlier period to 281 in the later period.
The information about grizzly bear deaths in Alberta from 1976 to 1988 and from 1988 to 2000 affects the prediction made in question 1 by providing additional data points and context.This decrease in grizzly bear deaths suggests a possible declining trend in the population. If the overall trend continues, it is reasonable to assume that the population has decreased further since 2000.
Taking this new information into account, the prediction made in question 1 should be revised. Instead of assuming a stable population or a slight increase, it would be more accurate to predict a decline in the grizzly bear population in Alberta. However, it is important to note that additional factors, such as conservation efforts or changes in habitat, should also be considered to gain a comprehensive understanding of the population dynamics.
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Plants containing only chlorophyll b are exposed to radiation with the following wavelengths: 10nm (X-rays), 450nm (blue light), 670nm (red light), and 800nm (infrared light). Which plants harness the most energy for photosynthesis?
The plant with blue light is absorbed by chlorophyll B and the plant gets a lot of photons for its photosynthesis. Because the blue lights, comes first in this peak of absorption.
Chlorophyll, is the primary pigment used in the process of photosynthesis, reflects green light and absorbs red and blue light most strongly. In plants, photosynthesis takes place in chloroplasts, which contain the chlorophyll.
In plants the color of the pigment comes from the wavelengths of light that are reflected, or the one those are not absorbed. For Chlorophyll, the green pigment is most common as it absorbs all wavelengths of visible light except green, which it reflects.
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In order to be transcribed, DNA molecules separate between which of the following parts?
A.sugars
B.phosphate groups
C.nitrogen bases
D.carbohytrates
Answer:
nitrogen bases
Explanation:
For transcription, the DNA molecules are separated between the option c. nitrogen bases.
What is transcription?It is the process when the DNA segment is copied to the RNA.
The DNA segments convert into the RNA molecules that encoded the proteins that generated the messenger RNA.
Based on this, we can say that For transcription, the DNA molecules are separated between the option c. nitrogen bases.
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Order the life cycle of very high-mass stars Nebula , protostar, red supergiant, supernova, average star and black hole ?
Can you breathe with a closed mouth? If yes how does air pass in?
Answer:
yes, through your nose
Explanation:
yes