cystic fibrosis is caused by nonsense and missense mutations in the cftr gene, which encodes for a chloride channel. you are studying cystic fibrosis patients to determine what mutation they possess in the cftr gene. the difference between the mutant and wild type cftr genes can be uncovered by examining the cftr:

Answers

Answer 1

Examining the function of the CFTR protein, such as its capacity to transport chloride ions across the cell membrane, reveals the distinction between the mutant and wild type CFTR genes.

Mutations in the CFTR gene, which encodes a protein that acts as a chloride channel, are the root cause of the genetic condition known as cystic fibrosis (CF). The CFTR gene can be affected by missense and nonsense mutations, which can result in the protein not functioning properly and the typical symptoms of CF. As a result, researchers can measure the CFTR protein's capacity to transport chloride ions in order to ascertain the specific mutation in the CFTR gene that a patient possesses.

Electrophysiology, ion-selective electrodes, and fluorescent dyes are just a few of the tools that can be used to accomplish this. Healthcare providers can tailor a patient's treatment to effectively manage CF symptoms by knowing the specific mutation.

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Related Questions

the function of a eukaryotic cell are managed by the

Answers

Each organelle and molecular process contributes to specific functions, such as energy production, protein synthesis, waste removal, and maintaining cellular homeostasis

The function of a eukaryotic cell is managed by a complex network of organelles, molecules, and processes. These components work together to ensure the cell's survival, growth, and proper functioning. Some of the key contributors to cellular function in eukaryotic cells include:

Nucleus: The nucleus contains the cell's genetic material and serves as the control center. It regulates gene expression, DNA replication, and provides instructions for protein synthesis.

Mitochondria: Mitochondria are the powerhouse of the cell, generating energy in the form of ATP through cellular respiration. They play a vital role in metabolism and provide energy for various cellular processes.

Endoplasmic Reticulum (ER): The ER is involved in protein synthesis, folding, and transport. The rough ER synthesizes proteins destined for secretion or insertion into cellular membranes, while the smooth ER is involved in lipid synthesis and detoxification.

Golgi Apparatus: The Golgi apparatus modifies, sorts, and packages proteins and lipids received from the ER. It prepares them for transport to specific cellular destinations or secretion outside the cell.

Lysosomes: Lysosomes contain digestive enzymes that break down cellular waste, damaged organelles, and engulfed particles. They play a role in recycling cellular components and maintaining cellular homeostasis.

Cytoskeleton: The cytoskeleton provides structural support to the cell and is involved in cell movement, shape determination, and intracellular transport. It is composed of microfilaments, intermediate filaments, and microtubules.

Plasma Membrane: The plasma membrane regulates the passage of substances in and out of the cell. It is involved in cell signaling, communication, and maintaining cellular integrity.

These are just a few examples of the many components involved in managing cellular function in eukaryotic cells. . The intricate coordination of these components ensures the overall functionality and survival of eukaryotic cells.

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A(n) _____ is a thick film of protein secreted by mucous membranes in the mouth that coats teeth and provides a substrate for Stretptococcus mutans to adhere and begin the formation of a slime layer.

Answers

Answer:

The correct answer is - the biofilm.

Explanation:

Biofilms are a type of one or more than one type of microbes that grow on many different surfaces, These microbes include fungi, bacteria, and protist that develop attached to the surface and one another and embedded in a self-produced matrix.

It is a thick film of protein such as fibrin, polysaccharides such as alginate or eDNA secreted by the bacteria, and other microbes that cover the teeth surface.

_____ stores chemical energy in just the right size for
transport and uptake by cells.
a. Glucose
b. ADP
c. ATP
d. Sucrose

Answers

Answer:

A. Glucose

Explanation:

Glucose stores chemical energy in just the right size for transport and uptake by cells.

Answer:

Glucose

Explanation:

sorry if I’m wrong.

why must dentures be relined periodically? group of answer choices dentures absorb odors from plaque and food. dimensional changes in acrylic occur. alveolar ridges shrink over time.

Answers

Dentures must be relined periodically for several reasons. One reason is that dentures can absorb odors from plaque and food, which can lead to bad breath.

Dentures come in two main types: complete dentures and partial dentures. Complete dentures are used when all teeth in the upper or lower jaw are missing, while partial dentures are used when some natural teeth still remain. Complete dentures consist of a full set of artificial teeth mounted on an acrylic base that fits over the gums. Partial dentures, on the other hand, have artificial teeth attached to a metal or acrylic framework that clasps onto the remaining natural teeth for support and stability.

The process of getting dentures typically involves multiple dental appointments. The dentist takes impressions of the patient's mouth to create a custom mold for the dentures. This ensures a proper fit and bite alignment. The dentures are then fabricated in a dental laboratory based on these impressions. Once the dentures are ready, the dentist performs any necessary adjustments to ensure a comfortable fit.

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If Plant A with yellow flower (dominant trait) is crossed with Plant B white flower (recessive trait), what is the percentage of the offspring having a yellow flower? Plant A: YY Plant B: yy

Answers

Answer:

100%

Explanation:

Yellow flower color (Y) is dominant over white flower color (y). Plant A is YY while plant B is yy:

       YY    x    yy

  Yy    Yy    Yy    Yy

All the offspring would have Yy genotype and since Y is dominant over y, all of them will have yellow flower color as opposed to while.

Hence, the percentage of the offspring that will have a yellow flower would be 100%.

On the non-working side in an ideal occlusion, interfering contacts on posterior teeth will be located on which inclines of which cusps.
- inclines
- Inner cusps
- Inner supporting

Answers

In an ideal occlusion on the non-working side, interfering contacts on posterior teeth will be located on the inclines of the inner cusps.

These inner cusps are important as they play a role in guiding the mandible during lateral movements.

Interfering contacts can lead to issues such as occlusal disharmony and potential tooth or TMJ problems.

To be more specific, on the non-working side, the interfering contacts will be found on the buccal inclines of the maxillary inner cusps and the lingual inclines of the mandibular inner cusps.

These inclines of the inner cusps serve as inner supporting structures during normal jaw movements, ensuring proper occlusal stability and preventing undue stress on the teeth and temporomandibular joint (TMJ).

Eliminating such interferences is crucial for maintaining a balanced and functional occlusion. Hence the posterior teeth will be located on the inclines of inner cusps.

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Fibronectin is the ligand of a particular adhesion molecule. Therefore, that adhesion molecule most likely belongs to which family?
A. Cadherins
B. Collagens
C. Immunoglobulin superfamily
D. Integrins
E. Selectins

Answers

Integrins are a family of transmembrane adhesion molecules that mediate cell-cell and cell-extracellular matrix interactions. The correct answer is D. Integrins.

Fibronectin is a high-molecular-weight glycoprotein that is present in the extracellular matrix and serves as a ligand for several integrins. Therefore, an adhesion molecule that binds to fibronectin is most likely an integrin.

Integrins play a crucial role in cell adhesion, migration, and signaling, and are involved in many physiological and pathological processes, including wound healing, inflammation, and cancer metastasis.

Integrins are composed of alpha and beta subunits that form a heterodimeric complex. The extracellular domain of the integrin binds to specific ligands, such as fibronectin, while the intracellular domain interacts with the cytoskeleton and regulates cell signaling pathways.

In contrast, cadherins are calcium-dependent adhesion molecules that mediate cell-cell interactions, collagens are a family of extracellular matrix proteins that provide structural support to tissues, immunoglobulin superfamily molecules are involved in immune responses, and selectins mediate cell-cell interactions during inflammation and immune responses.

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Species that form large toxic blooms in the ocean are:.

Answers

Answer:

Species that form large toxic blooms in the ocean are... dinoflagellates.

Find the length of the diagonal of a square whose side
is 10 cm​

Answers

Answer:

14.14 cm

Explanation:

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draw the structure of the tetrahedral intermediate initially formed in the reaction shown.

Answers

The tetrahedral intermediate is initially formed during the nucleophilic attack on the carbonyl carbon in a reaction. This intermediate is a transient species, meaning that it is very reactive and has a short lifespan. The tetrahedral intermediate has a tetrahedral shape, and the carbon atom in the center of the tetrahedron is sp3 hybridized.

The four atoms that are bonded to the central carbon are arranged in a tetrahedral geometry. The hybrid orbitals of the central carbon atom overlap with the orbitals of the nucleophile and the carbonyl oxygen atom to form new covalent bonds. The tetrahedral intermediate is formed before it collapses and re-forms the carbonyl functional group.

The structure of the tetrahedral intermediate initially formed in the reaction shown can be represented as shown below: \(\ce{R-C(H)=O + HNR2 -> R-C(H)(NR2)-O-H}\) where R represents an alkyl or aryl group. The tetrahedral intermediate formed after nucleophilic attack by the amine molecule.

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Which type of symbiosis is the
relationship between plants and nitrogen
fixing bacteria?
Parasitism
Commensalism
Mutualism
Predation

Answers

Answer:

mutualism

Explanation:

Both benefit: bacteria get food (glucose and nutrients) while plants get usable forms of nitrogen.

Mutualism both benefit from each other

Which part of the plant cell does photosynthesis occur? Please help

Answers

Answer:

Photosynthesis occurs in the chloroplast of the plant cell.

Explanation:

Some events that take place during protein synthesis are listed.
1. DNA serves as a template for RNA production
2.Transfer RNA binds to specific amino acids and carries them to the ribosomes
3. Amino acids are bonded together to make proteins
4. Messenger RNA moves from the nucleus to the ribosomes
What is the correct order of protein synthesis?
3-2-4-1
1-4-2-3
1-2-4-3

Answers

I beleive the answer is 1-4-2-3. I did this a while ago in edge but it was worded differently.

Why KOH solution is kept in the test tube inside the air tight conical flask while doing the experiment of respiration of seeds?

Answers

Answer:

It is kept inside to absorb the CO2 released by the germinating seeds.

Explanation:

It means it will create a cycle for the flask. So, an equal volume of water rises up in the tube.

Hope it helps.

When oceanic and continental plates converge, the oceanic plate tends to go under the continental plate and eventually melts. Which feature below forms at these types of boundaries?

A) rift valley

B) seafloor spread

C) volcanic mountains

D) folded mountains

Answers

I believe it is C- volcanic mountains

When oceanic and continental plates converge, the oceanic plate tends to go under the continental plate and eventually melts. Volcanic mountains forms at these types of boundaries. Thus, option C is correct.

How are mountains formed?

Mountains are often formed by the movement of tectonic plates on the earth's crust. The crust itself is made by the different large tectonic plates. There are three ways to form a mountain which is following, volcanic, fold, and block mountains.

The great African rift valley and Rhine valley in Germany is an examples of block mountains. Fold mountains are the Himalayas, Alps, and the Rockies. Volcanic mountains are Vesuvius, Kilimanjaro, and Mount Fuji.

"At a divergent boundary in a continental plate" is the one among the following choices given in the question that tells where a rift valley is most likely to occur.

Therefore, When oceanic and continental plates converge, the oceanic plate tends to go under the continental plate and eventually melts. Volcanic mountains forms at these types of boundaries. Thus, option C is correct.

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Bacteria are capable of
reproducing quickly. Explain how
this adaptation is beneficial in
their ability to become resistant
to antibiotics

Bacteria are capable ofreproducing quickly. Explain howthis adaptation is beneficial intheir ability

Answers

Answer:

Antibiotics, also known as antimicrobial drugs, are drugs that fight infections caused by bacteria. Alexander Fleming discovered the first antibiotic, penicillin, in 1927. After the first use of antibiotics in the 1940s, they transformed medical care and dramatically reduced illness and death from infectious diseases.

The term "antibiotic" originally referred to a natural compound produced by a fungus or another microorganism that kills bacteria which cause disease in humans or animals. Some antibiotics may be synthetic compounds (not produced by microorganisms) that can also kill or inhibit the growth of microbes. Technically, the term "antimicrobial agent" refers to both natural and synthetic compounds; however, many people use the word "antibiotic" to refer to both. Although antibiotics have many beneficial effects, their use has contributed to the problem of antibiotic resistance.

Antibiotic resistance is the ability of bacteria or other microbes to resist the effects of an antibiotic. Antibiotic resistance occurs when bacteria change in some way that reduces or eliminates the effectiveness of drugs, chemicals, or other agents designed to cure or prevent infections. The bacteria survive and continue to multiply causing more harm.

how many oxygen atoms are present in a formula unit of calcium acetate?

Answers

Four carbon atoms, six hydrogen atoms, one calcium atom, four oxygen atoms, and one calcium atom make up the calcium acetate formula unit.

Calcium acetate has the chemical formula C4H6CaO4 at the molecular level.Patients with end-stage kidney disease who are receiving dialysis can use calcium acetate to treat hyperphosphatemia (too much phosphate in the blood).

For patients receiving dialysis due to severe kidney disease, calcium acetate is a calcium supplement used to regulate the level of phosphate in the blood. The following brand names for calcium acetate are also available: Eliphos, PhosLo, Phoslyra, and Calphron.

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Why is an alteration of electrophoretic mobility interpreted as a change in the primary structure of the protein under study

Answers

Electrophoretic mobility refers to the rate at which a charged molecule, such as a protein, moves through an electric field during gel electrophoresis.

Proteins with different primary structures and charge distributions will exhibit different electrophoretic mobilities. Therefore, an alteration in the electrophoretic mobility of a protein under study can be interpreted as a change in its primary structure, such as the addition or removal of amino acids or post-translational modifications. This technique is commonly used to analyze proteins and their modifications, providing valuable information about protein function, structure, and interactions. However, it should be noted that changes in electrophoretic mobility can also be due to other factors, such as changes in pH or buffer conditions.

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the disappearance of _________ junctions may occur when cancer cells metastasize.

Answers

The disappearance of tight junctions may occur when cancer cells metastasize.

What is Tight junction?

Tight junctions are a type of cell-cell junction that helps to keep cells together and prevent the passage of molecules between them. When tight junctions are disrupted, it can allow cancer cells to escape from the original tumor and spread to other parts of the body.

Factors that can contribute to the disappearance of tight junctions, are:Changes in the expression of tight junction proteins: Cancer cells can sometimes change the expression of the proteins that make up tight junctions. This can weaken the junctions and make them more likely to break down.Invasion of the extracellular matrix: Cancer cells can also invade the extracellular matrix, which is the material that surrounds cells. This can damage tight junctions and make it easier for cancer cells to escape.Inflammation: Inflammation can also damage tight junctions. This is because inflammation can increase the production of enzymes that break down the proteins that make up tight junctions.

The disappearance of tight junctions is a major step in the process of cancer metastasis. By breaking down the barriers that keep cells together, cancer cells can escape from the original tumor and spread to other parts of the body. This can make cancer much more difficult to treat.

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3. The radula and crystalline style of molluscs are part of the:
A) reproductive system
B) defensive mechanisms
C) excretory system
D) nervous system
E) digestive system

Answers

Answer:

E. digestive system

Explanation:

Mollusks/Molluscs are invertebrates of the Kingdom Animalia. It is said to have the biggest population when it comes to the marine phylum.

It has an organ called the "radula" that is responsible for grinding food. This organ looks like a tongue. Its "crystalline style" is located in the middle part of its gut. It is responsible for secreting enzymes and it helps in extracellular digestions.

Clearly, the radula and crystalline style are both part of the mollusc's digestive system.

explain the meaning of the term species​

Answers

Species is defined as a group of living organisms that have similar characteristics genetically and are capable of interbreeding among themselves.

A biological species is a group of organisms that can reproduce with one another in nature and produce fertile offspring. Species are characterized by the fact that they are reproductively isolated from other groups, which means that the organisms in one species are incapable of reproducing with organisms in another species. The term species can also be defined as the most basic category in the system of taxonomy. Taxonomy is a scientific system that classifies organisms based on their biological characteristics. Species can also be defined based on a shared evolutionary history and ancestry. This method of defining species is called phylogenetics, which is the study of the evolutionary relationships among organisms. The evolutionary process by which a new species comes into being is called speciation.

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studies done by buchner showed that ground-up yeast cells were able to convert sugar to alcohol. the components of the mixture that were responsible for this transformation were

Answers

Enzymes invertase, zymase and diastase were the particular enzymes in the combination that were responsible for this transformation, according to Buchner's investigations, which demonstrated that ground-up yeast cells were capable of converting sugar to alcohol.

Zymase and diastase further break down the sugar molecules into alcohol and carbon dioxide after invertase has converted them into smaller molecules.

The DNA molecules in the yeast cells make the enzymes, which are a sort of protein. Although the combination also contains lipids and carbs, neither are necessary for the transformation of sugar into alcohol.

While carbohydrates are essential for the creation of cell structure and membranes, lipids are primarily employed as an energy source.

Complete Question:

Studies done by Buchner showed that ground-up yeast cells were able to convert sugar to alcohol. The components of the mixture that were responsible for this transformation were

DNA molecules.

enzymes.

lipids.

carbohydrates.

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your professor wants you to construct a phylogenetic tree of a type of plant called orchids. she gives you tissue from seven orchid species and one lily plant. what is the most likely reason she gave you the lily?

Answers

The most likely reason your professor provided you with the lily plant tissue, in addition to the seven orchid species, is to use it as an outgroup in constructing the phylogenetic tree.

An outgroup serves as a reference point, helping to determine the evolutionary relationships among the ingroup taxa, in this case, the orchid species.

In a phylogenetic tree, an outgroup is a species or group of species that is closely related to, but not a member of, the group of species under study (the ingroup). By comparing the characteristics of the ingroup taxa with the outgroup, you can identify shared traits and better infer the evolutionary history of the species in question. The lily, being a related but distinct plant, can help establish the relative branching order and ancestral characteristics of the orchids in the phylogenetic tree.

Using the lily as an outgroup will make your analysis more robust, allowing you to build a more accurate and informative phylogenetic tree of the orchid species. Remember to be careful when selecting the appropriate outgroup, as it should be closely related to the ingroup but not so distant that homoplasy (convergent evolution) becomes an issue.

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is it likely that rna molecules functioned as ribozymes to synthesize dna from aminoacids, and that this role was reversed when dna became the information source?

Answers

RNA molecules do not synthesize DNA from amino acids, but they can serve as ribozymes in the reverse transcription of RNA to DNA, and they play a crucial role in protein synthesis as part of the ribosome.

The RNA molecule acts as a template for synthesizing DNA through reverse transcription, which is an RNA-dependent DNA synthesis reaction. RNA molecules may also serve as ribozymes in this scenario. Ribozymes are RNA molecules that function as enzymes, catalyzing various chemical reactions in the cell, just like enzymes made up of proteins. Some ribozymes can use RNA templates to synthesize new RNA molecules, while others can use RNA templates to synthesize DNA molecules.Since DNA contains genetic material and information, it became the primary source of genetic information in organisms, while RNA remained involved in catalyzing biochemical reactions. The RNA world hypothesis suggests that RNA preceded DNA in early life on Earth, serving both as genetic material and a catalyst for the formation of other molecules necessary for life.

The discovery of ribozymes has provided evidence that RNA may have played an even more prominent role in early life than previously thought. RNA molecules do not synthesize DNA from amino acids. Instead, ribosomes, which are made up of RNA and proteins, synthesize proteins from amino acids. RNA templates are used by ribosomes to direct the assembly of amino acids into the appropriate order to produce a functional protein. In summary, RNA molecules do not synthesize DNA from amino acids, but they can serve as ribozymes in the reverse transcription of RNA to DNA, and they play a crucial role in protein synthesis as part of the ribosome.

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Which of the following is NOT a characteristic shared by all prokaryotes and
eukaryotes?
a. They contain DNA in the nucleus of the cell
b. They have ribosomes that make proteins
c. The have enzymes that help to break down materials
d. They grow and develop

Answers

Answer: A. They contain DNA in the nucleus of the cell

Explanation: I just looked it up and I got that answer.

Amino acid is to protein as

Answers

Monicdoe is to scratch

how many checkpoints are there in mitosis

Answers

Answer:

3

Explanation:

hope that helps!!!!!!!

Answer:

three

Explanation:

There are three major checkpoints in the cell cycle: one near the end of G1, a second at the G2/M transition, and the third during metaphase.

State whether each water pollutant listed below is primary or secondary and whether it is physical or chemical biological radiological or thermal

State whether each water pollutant listed below is primary or secondary and whether it is physical or

Answers

Among the frequent contributors of water contamination include sewage disposal, industrial chemical spills, agricultural runoff, etc.

What are the primary and secondary water pollutant?

A secondary pollutant is created when other pollutants (primary pollutants) react with the atmosphere, rather than being directly released as such.

The primary reasons of water pollution are microorganisms, parasites, insecticides, pharmaceuticals, plastics, fecal matter, radioactive matters, fertilizers, and pesticides.

Therefore, Pollutants, both primary and secondary. A primary pollutant is a substance that is released into the air directly from a source.

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what’s the size of the smallest object that can be resolved by a visible-light (use green) microscope?

Answers

The smallest object that a visible-light microscope can discern using green light is around 200 nanometers in size.

The diffraction limit, which is governed by the wavelength of light employed and the numerical aperture (NA) of the lens system, determines the smallest object that can be resolved by a visible-light microscope.

Green light has a wavelength of roughly 550 nanometers (nm), or 0.55 micrometres (m) in visible light. The potential resolution limit for a green-light microscope is around 200 nanometers, or 0.2 micrometres, assuming a high-quality lens with a NA of 1.4.

This indicates that the smallest object that a visible-light microscope can discern using green light is around 200 nanometers in size. In practise, however, other factors such as lens aberrations and sample preparation might alter a microscope's true resolution. As a result, reaching the diffraction limit of resolution is not always feasible.

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10. Which type of muscle is responsible for a person jumping up and down? *

Answers

Answer:

Skeletal Muscle are the Muscles

of muscle is responsible for a person jumping up and down.

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