During the menstrual cycle, which of the following step occur first?
A) The uterine lining thickens, and ovulation.
B) There is menstrual discharge.
C) An egg moves to the uterus
D) The lining of the uterus breaks down
PLEASE HELP AND HURRY

Answers

Answer 1

Answer:

A I think

Explanation:

Answer 2
I think the answer is letter B
Because the first one is the last step, the 3rd one is ovulation and the fourth one is Follicular stage I’m pretty sure
-sorry if wrong

Related Questions

what is a advantages of dialysis

Answers

Answer:

The answer is at the bottom!!

Explanation:

In comparison to PD, one of the major benefits of hemodialysis is that it enables patients to have four days per week where treatment is required. This arguably can further improve patients' quality of life by giving them more freedom.

Hope this helps!!

Answer:

life quality Sometimes people with kidney problems are unable to continue living their lives comfortably because of their condition.

Explanation:

Where is most of the baryonic matter (ordinary matter) of the universe found?

A. in planets and natural satellites
B. in comets and asteroids
C. in dark matter and dark energy
D. in interstellar gases and stars

Answers

Baryonic dark matter may occur in non-luminous gas or in Massive Astrophysical Compact Halo Objects, or stars. so therefore the answer is D.

Most of the baryonic matter (ordinary matter) of the universe are found in

planets and natural satellites.

The baryonic matter is composed of particles present in the nucleus such as

the proton and neutron . Electrons or other sub-particles aren't present in

the baryonic matter.

This type of matter is present in stars with a percentage of about 10 percent

and in planets and satellites with a higher percentage.

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3. Why are solar prominences unable to leave the sun's atmosphere?
Their arches are very insignificant.
Gravity draws them back in.
They occur for only a few seconds.
The electric charges easily reach humans on Earth.

Answers

The core dynamo of the sun creates a complex network of magnetic fields along which the prominence plasma moves. When such a structure loses stability, it explodes outward, expelling the plasma, and this is known as an erupting prominence.

what is solar prominences?  

   A solar prominence is a sizable, luminous feature that extends from the Sun's surface and is also referred to as a filament when seen against the solar disk. The photosphere, where prominences are tethered to the Sun's surface, extends into the corona, the Sun's scorching outer atmosphere. A prominence develops over the course of about a day, and stable prominences can arc hundreds of thousands of kilometers into space for several months while remaining in the corona. Researchers are still trying to understand how and why prominences develop

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PLS ANSWER!!!!!!!!!!!!!!!!!!!

PLS ANSWER!!!!!!!!!!!!!!!!!!!

Answers

Answer:

Molecules of carbon dioxide (CO 2) can absorb energy from infrared radiation.

Explanation:

plants release water vapor through their leaves via a process called ________. a. transpiration b. percolation c. condensation d. evaporation

Answers

The process through which plants release water vapor through their leaves is known as transpiration. This process plays a significant role in the water cycle.

The process through which plants release water vapor through their leaves is known as transpiration. This process plays a significant role in the water cycle. Explaining water vapor, transpiration and the significance of the process in the water cycle is given below: Water vapor is the gaseous state of water. When water molecules evaporate from a surface, they turn into water vapor. As the vapor rises in the atmosphere, it cools and condenses, forming clouds. When the clouds become too heavy with water droplets, they fall as precipitation in the form of rain, sleet, or snow. Transpiration is the process through which plants release water vapor into the atmosphere. It is the evaporation of water from plants through the stomata on their leaves. Transpiration helps in the uptake of water from the roots of the plant and also helps to regulate the temperature of the plant. Water vapor released through transpiration cools the leaf surface and the surrounding environment. Significance of transpiration in the water cycle: The process of transpiration plays a significant role in the water cycle. When water vapor is released into the atmosphere through transpiration, it contributes to the moisture in the atmosphere. The moisture eventually condenses and falls back to the earth as precipitation. This cycle of transpiration, evaporation, and precipitation ensures a constant supply of water to sustain life on earth. Thus, transpiration is an important part of the water cycle.

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6 reasons why Meiosis and mitosis are different (PLEASE HELP)

Answers

Answer:

Reasons why they're different :

Explanation:

1. Mitosis is reserved for somatic cells while meiosis is reserved for sex cells (gametes).

2. Mitosis results in identical cells , while meiosis results in genetically distinct cells.

3. Mitosis undergoes one round of cell division while meiosis undergoes two rounds of divison.

4. Mitosis results in diploid daughter cells, while meiosis results in haploid daughter cells.

5. Unlike mitosis, meiosis differs in males and females.

6. Mitosis creates a human cell with 46 chromosomes, while meiosis creates a human cell with 23 chromosomes.

Why is the Earth able to escape the Sun gravitational field?

a) The amount of gravity has decreased in the system and now the Sun doesn’t have enough pull to keep the Earth in orbit.

b) The Earth distance is greater and therefore the gravity is less.

c) The little blue arrows got so small - something must have changed.

Can’t find Science for subjects.

Answers

b

Explanation:

The Sun's gravity is enough to keep the Earth from flying off in a straight line, away from the Sun, but not enough to bring the Earth closer in - the Earth is continually changing its direction of movement, but in such a way that it follows a nearly circular path around the Sun.

Describe the role of the hippocampal circuits in long-term
memory formation (1000 words)

Answers

Memory development is significantly influenced by the complex network of hippocampal circuits and connections with other brain areas.

Encoding of memories involves sensory information and experiences being processed in the neocortex, which is then transmitted to the hippocampus through pattern separation.

The hippocampus provides contextual and sensory information, facilitating the retrieval of associated memories. The hippocampus also aids in spatial navigation, with place cells in the CA1 region exhibiting activity patterns specific to specific locations in an environment.

The interplay between grid cells, place cells, and head-direction cells enables accurate perception and navigation through space. In conclusion, the hippocampal circuits play a vital role in long-term memory formation, transforming transient experiences into lasting memories, providing the foundation for learning and remembrance.

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which of these declarations is accurate concerning the life cycle of a pine tree?

Answers

The statement about the pine life cycle that is accurate conifer pollen grains contain male gametophytes, the correct option is (D).


Pine trees, like other conifers, have a life cycle that involves the production of male and female gametophytes. In conifers, the male gametophytes are contained within the pollen grains. These pollen grains are produced in the male cones and are released into the air. When the pollen grains reach the female cones, they undergo pollination. Each pollen grain contains male gametes (sperm cells) that will fertilize the female gametophyte.

The pine tree is not a gametophyte itself but a sporophyte, which is the dominant generation in the pine life cycle. Male and female gametophytes are not in close proximity during gamete synthesis because pollen grains are released into the air and can travel long distances. Double fertilization, which occurs in angiosperms, is not a characteristic of the pine life cycle, the correct option is (D).  

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The complete question is:


Which of the following statements about the pine life cycle is accurate?

A) The pine tree is a gametophyte.

B) Male and female gametophytes are in close proximity during gamete synthesis.

C) Double fertilization is a relatively common phenomenon.

D) Conifer pollen grains contain male gametophytes.

What adaptation do seeds dispersed through ballistochory have?
a)

sail-like structure

b)

smooth and aerodynamic

c)

hollow on the inside

d)

pleasant smell and taste

Answers

Answer:

smooth and aerodynamic

Explanation:

Seeds are usually smooth, like a baseball, so that they cut smoothly through the air and don't have a lot of wind resistance.

The adaptation that seeds dispersed through ballistochory has smooth and aerodynamic. The correct option is b.

What are adaptations?

Adaptation is a biological process that is done by many plants and animals to survive in given conditions. Adaptation is causing the partial or full change in their body or functions to match with the environmental conditions.

Plants with ballistochory means possibly ejects by the seeds full stop this adaptation or evolution happened in which seats are dispersed into many tentacles and seats are dispersed into soils for pollination this comes under wind dispersion.

Seeds launch an angle and release the elastic energy store in the fruit. These adaptations are smooth.

Therefore, the correct option is b. smooth and aerodynamic

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Which traits separate fungi from plants?

Answers

We now know that they have unique physical, chemical, and genetic traits that set them apart from plants and other eukaryotes. For example, the cell walls of fungi are made of chitin, not cellulose. Also, fungi absorb nutrients from other organisms, whereas plants make their own food.

Answer:

Fungi don't have chlorophyll and cannot produce their own food, the bodies of fungi are made of masses of filaments woven together, the fungal cell walls contain chitin complex polysacharide.

Explanation:

I NEED HELP QUICK Why can we see Codominance more in flowers that in people?

Answers

each gene came in just two different versions, ... We can see an example of codominance in the MN blood groups of humans (less ... Multiple Alleles are three or more possible alleles for one individual trait.

Select the correct answer. Which muscle is the woman using to lift the ball over her head? A basketball player lifting a ball over her head A. Biceps B. Deltoid C. Extensor digitorum D. Gluteus maximus E. Triceps

Answers

Answer:

B. Deltoid

Explanation:

The deltoid muscle is a thick muscle on the human shoulder. When the basketball player is lifting the ball, the shoulder muscle is being used to lift over her head.

Answer:

Got 100% right on plato!

Explanation:

Select the correct answer. Which muscle is the woman using to lift the ball over her head? A basketball

When plate tectonic processes closed off the Mediterranean Sea from the Atlantic ocean during the Late Miocene, species went extinct because the ______.

Answers

When plate tectonic processes closed off the Mediterranean Sea from the Atlantic Ocean during the Late Miocene, species went extinct because the Mediterranean Sea became more saline.

The closure of the Strait of Gibraltar, which occurred as a result of plate tectonic processes during the Late Miocene, caused the Mediterranean Sea to become more isolated from the Atlantic Ocean. This isolation led to a decrease in the amount of freshwater entering the Mediterranean, causing the sea to become more saline over time. This change in salinity had a profound impact on the marine organisms living in the Mediterranean, many of which were not adapted to survive in such high salinity conditions.

As a result, a number of species went extinct, while others evolved adaptations to the new environment. This event is an example of how changes in the Earth's geology and climate can have significant impacts on the evolution and extinction of species over time.

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4. Use the atomic model below to answer the following questions: 1 pt for each blank





How many protons?


How many neutrons?


What is the name of this atom?

4. Use the atomic model below to answer the following questions: 1 pt for each blankHow many protons?How

Answers

Answer:

for first 1 its 3 nuetros i think

The first is 7

The second is 8

And the last is 27

what is the probable sequence in which the following animal clades originated, from earliest to most recent? what is the probable sequence in which the following animal clades originated, from earliest to most recent? bilaterians, deuterostomes, amniotes, vertebrates, tetrapods

Answers

The probable sequence in which the following animal clades originated, from earliest to most recent, is as follows:
Vertebrates Deuterostomes Bilaterians Tetrapods Amniotes

This is a long answer because it provides a specific sequence and explanation for each clade.
The probable sequence in which the following animal clades originated, from earliest to most recent, is as follows: Bilaterians Deuterostomes Vertebrates Tetrapod. Amniotes

This sequence shows the progression of animal evolution, starting with the development of bilateral symmetry in bilaterians, followed by the deuterostomes which include chordates, then the emergence of vertebrates with a backbone, the transition to land-dwelling tetrapods, and finally the development of amniotes which are able to reproduce with an amniotic egg.

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3. Cardiac muscle cells make up which type of muscles?
(5 Points)
O A. Voluntary
B. Smooth Muscle Cells
C. Skeletal Muscle Cells
O D. Involuntary

Answers

Explanation:

voluntary muscles are ones that work without you having to think about it..example heart, lungs. part of the APT system...Autonomic ...

Onepage review on why understanding gas and vapors in a work setting is important and what we can do in our workplace to prevent accidents with gases and vapors.

Answers

Understanding gas and vapours in a work setting is incredibly important for workplace safety. Gas and vapours can contain noxious substances that, if exposed to, can lead to serious injury or health implications.

In order to prevent such accidents, employers must perform risk assessments in order to assess and identify any areas of risk and put in place control measures to limit them. If gas and vapours are used onsite, then suitable ventilation and extraction systems should be in place.

Employers should also ensure that their staff are aware of the potential risks associated with such substances and provide adequate training and instruction in how to work safely. Finally, employers should also check that any necessary personal protective equipment is provided and communications are in place to be able to notify relevant emergancy responders in case of an accident.

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how does the extraction procedure differ when the organic phase

Answers

The extraction procedure differs when the organic phase is involved. The procedure involves separating the desired organic compound from a mixture by transferring it into an immiscible organic solvent.

Extraction is a common technique used in chemistry to separate and purify substances from complex mixtures. In a typical extraction procedure, a solvent is used to selectively dissolve the desired compound while leaving impurities behind. When the organic phase is involved, the procedure takes advantage of the immiscibility between organic solvents and aqueous solutions.

The organic phase refers to the solvent that is primarily composed of organic compounds, such as ethers, alcohols, or hydrocarbons. To perform the extraction, the mixture containing the desired compound is combined with the organic solvent. Due to their immiscibility, the organic solvent forms a separate layer, which can be easily separated from the aqueous phase.

The target compound selectively partitions into the organic phase, allowing for its isolation and subsequent purification. This extraction procedure differs from other types of extractions, such as liquid-liquid extractions using aqueous solvents, where the desired compound would be in the aqueous phase. By utilizing an organic phase, specific organic compounds can be efficiently extracted and separated from complex mixtures.

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Which statement best explains why breaks of finches in the Galapagos islands are different based on what to eat? A. Different organisms interact with each other. B. Individual parts work together to perform a function. C. Structure and function are tied together or related in living things. D. All life must maintain homeostasis in older to survive Please help me

Answers

Answer:

Explanation: A. Different organisms interact with each other

A dietician asks a patient about the food that the patient eats and makes the table below to summarize the results.

Macromolecule
Suggested percentage of diet
Actual percentage of diet
Carbohydrates 45–65% 70%
Lipids 20–35% 5%
Proteins 10–35% 25%

Based on the table, what advice do you think that the dietician will give the patient?

Answers

Answer: The answer is C.

Explanation:

the patient is eating to much carbohydrates and not enough lipids.

The dietician will advise the patient to decrease rice and pasta in their diet and to increase the lean meat to have a balanced diet. Thus, option D is correct.

Who is a dietician?

A dietician is a person that is an expert in nutrition and diet. They provide the details and charts about the diet and food one should consume to have a balanced diet and nourishment.

As the percentage of carbohydrates is more in the diet the dietician will suggest decreasing the rice and pasta intake and increasing the protein percentage by increasing the intake of lean meat.

Therefore, dieticians will suggest decreasing carbohydrates and increasing proteins.

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The complete question is attached to the image below.

A dietician asks a patient about the food that the patient eats and makes the table below to summarize

I need help with this question

I need help with this question

Answers

Answer:

The optimal fermentation temperature of traditional brewing yeast is 28–33°C, generally no more than 36°C

Cup c will have a lot of bubbly water as the yeast produces carbon dioxide

Explanation:

What is the implication of the different classification of cells. Is it necessary to sustain life? Why? ​

Answers

Answer:A: "Where's Anna?" - B: "She's not here. She's over __________." *

1 điểm

there

here

out

in

Look at the sky! It__________. *

1 điểm

is going to rain

will rain

rains

is raining

We__________ a new car soon. *

1 điểm

get

will get

are getting

are going to get

He made __________ a sandwich. *

1 điểm

itself

himself

herself

yourself

A: "Would you like to come over for dinner?" - B: "__________." *

1 điểm

I'd love to but I have a lot of homework tonight

You're welcome

There are lots of drinks, food and desserts

I'm fine. Thank you.

A: "Let's make a new poster for our project." - B: " __________." *

1 điểm

Sure

That sounds great

Good idea

All are correct

We have a ____________ house. *

1 điểm

nice small old green

green old small nice

small nice old green

old green nice small

The children are playing _________. They are in the garden. *

1 điểm

inside

upstairs

outside

downstairs

Class *

Come in and have a seat! Help __________, children! *

1 điểm

itself

yourselves

ourselves

themselves

I am not __________ to play basketball. *

1 điểm

enough tall

tall enough

too tall

tall too

Explanation:

do cells produce waste

Answers

no they do not your welcome

continuous sequence of growth (interphase) and division (mitosis) in a cell. cell growth phase where a cell increases in size, carries on metabolism, and duplicates chromosomes prior to division. period of nuclear cell division in which two daughter cells are formed, each containing a complete set of chromosomes.

Answers

The cell cycle is a four-stage procedure in which the cell enlarges replicates its DNA  gets ready to divide ( and splits  (mitosis, or M, stage).

What is the cell's continuous process of growth, interphase, and division during mitosis?The cell cycle is a four-step process in which the cell grows in size (gap 1, or G1, stage), duplicates its DNA (synthesis, or S, stage), gets ready to divide (gap 2, or G2, stage), and divides (gap 3, or S, stage) (mitosis, or M, stage). Interphase, which accounts for the time between cell divisions, is made up of the phases G1, S, and G2.Interphase. The cell prepares for cell division during interphase while simultaneously going through regular activities. Numerous internal and external requirements must be satisfied for a cell to transition from the interphase to the mitotic phase.

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Distinguish between epigeal and hypogeal germination.
Three points please. It gets me confused ‍♀️

Answers

Answer:

The main difference between epigeal and hypogeal germination is that in epigeal germination, the cotyledons emerge out of the soil during germination whereas, in hypogeal germination, the cotyledons remain inside the soil.

you are interested in how cyclic-amp-dependent protein kinase a (pka) functions to affect learning and memory, and you decide to study its function in the brain. it is known that, in the cells you are studying, pka works via a signal transduction pathway like the one depicted in figure 16-15. furthermore, it is also known that activated pka phosphorylates the transcriptional regulator called nerd that then activates transcription of the gene brainy. which situation described below will lead to an increase in brainy transcription?

Answers

The situation that will lead to an increase in Brainy transcription is a mutation in the Nerd gene that produces a protein that cannot be phosphorylated by PKA. Option C is correct.

PKA activation is essential for phosphorylating the transcriptional regulator Nerd, which in turn activates the transcription of the Brainy gene. Therefore, if there is a mutation in the Nerd gene that produces a protein unable to be phosphorylated by PKA, it would disrupt the activation of Nerd and impair its ability to initiate Brainy gene transcription.

In contrast, the other options mentioned, such as mutations in adenylyl cyclase, PKA nuclear import sequence, or CAMP phosphodiesterase, would likely interfere with the cAMP-dependent signaling pathway or PKA activation itself, potentially leading to decreased PKA activity and subsequent effects on Brainy transcription. Option C is correct.

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The complete question is:

You are interested in how cyclic-AMP-dependent protein kinase A (PKA) functions to affect learning and memory, and you decide to study its function in the brain. It is known that, in the cells you are studying, PKA works via a signal transduction pathway like the one depicted in the figure to the right. Furthermore, it is also known that activated PKA phosphorylates the transcriptional regulator called Nerd that then activates transcription of the gene Brainy. Which situation described below will lead to an increase in Brainy transcription?

A. a mutation in the gene that encodes adenylyl cyclase that renders the enzyme unable to interact with the a subunit of the G protein

B. a mutation in the nuclear import sequence of PKA that makes it hydrophilic

C. a mutation in the Nerd gene that produces a protein that cannot be phosphorylated by PKA

D. a mutation in the gene that encodes CAMP phosphodiesterase that makes the enzyme inactive

You ate a Costco size bag of salty chips called nacho doritos® (Yay! I'm not alone!). Which of the following is true? O aldosterone → sodium channels on descending loop of Henle → Na+ reabsorption O aldosterone → sodium channels on collecting duct Na+ reabsorption O aldosterone → sodium channels on distal convoluted tubule → Na+ reabsorption O aldosterone sodium channels on ascending loop of Henle → | Na+ reabsorption

Answers

Aldosterone sodium channels on distal convoluted tubule → Na+ reabsorption is true.

Aldosterone is a hormone produced by the adrenal gland that regulates sodium and water balance in the body. When sodium levels are low, aldosterone is released. As aldosterone is released it then activates sodium channels in the distal convoluted tubule of the nephron in the kidney. This allows for increased reabsorption of sodium, which helps to maintain proper electrolyte balance in the body. So, after eating a large number of salty chips, the release of aldosterone would lead to increased sodium reabsorption in the distal convoluted tubule of the nephron.

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For those individuals who have an allergic reaction to cats, a company in Los Angeles promises relief. They offer a new line of
cats genetically modified to eliminate or reduce their allergy-causing properties. The development of this new line of cats most
likely involved
(1) using natural selection to produce a new variety of cat (3) changing the behavior of cats
(2) altering the reproductive rate of cats
(4) manipulating the DNA of cats

Answers

Answer:

1 or 4..

Explanation:

Answer:

Answer is D. Altering the DNA of cats

Explanation:

identify the enzymes that are required for the synthesis of a glycogen particle starting from glucose 6‑phosphate.

Answers

Enzymes involved in glycogen synthesis from glucose-6-phosphate are Hexokinase, Phosphoglucomutase, Glycogen Synthase, and Branching Enzyme.

Glucose-6-phosphate is the precursor molecule for glycogen synthesis. Four enzymes are needed to make a glycogen particle from glucose 6-phosphate. They are Hexokinase, Phosphoglucomutase, Glycogen Synthase, and Branching Enzyme. Hexokinase catalyzes the phosphorylation of glucose to glucose 6-phosphate. Phosphoglucomutase is responsible for the conversion of glucose 6-phosphate to glucose 1-phosphate.

Glycogen Synthase is the key enzyme that converts glucose 1-phosphate to glycogen. It creates alpha-1,4-glycosidic bonds between glucose molecules. Branching Enzyme is responsible for creating branch points in glycogen. It creates alpha-1,6-glycosidic bonds. These enzymes work together to produce glycogen. Glycogen is stored in liver and muscle cells and is used as a source of energy during times of fasting and exercise.

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In your own word pls based on the story What two things should be in your description of a story's theme?a.what the theme is and why you think sob.what the theme is and who the characters arec.when and how you figured out the themed.the parts of the plot that show theme, and the conflict We've talked a lot about "fixed" and "variable" expenses. We've talked about the way these expenses impact your budget and your savings. We also talked about one big thing you can do about variable expenses that you cannot usually do about your fixed expenses. What was the big thing you can do? How does Makkah create the tone of The Briefcase ? Nicole was arrested for stealing a television from her neighbor. She was fined $50,000 and sentenced to 10 years in jail. She is suing the government because she thinks her rights were violated. Which amendment to the US Constitution contains the specific right that was violated?Tenth AmendmentEighth AmendmentFifth AmendmentSixth Amendment there is a strong positive correlation between ice cream sales and instances of snakebites. which of the following is the most likely explanation for this correlation? International marketers and advertisers face challenges not encountered domestically. The most basic issue concerns ____________. Based on the table, what is the unit rate for one gallon of gasoline? Locate a film noir stylistic trait in the mise-en-scene (again: this could be anything from lighting, to acting, to proxemics, to set or costume design) of a contemporary film and discuss its significance. Use a screenshot that you think represents this characteristic well. Please think seriously about the characteristics of noir (i.e. these do not appear in every film). 20 points!~ And thanks if you help me. (v) List down two factors that cause differences in the temperature range between these cities. where did the allies use the atomic bomb in japan What is genetic biodiversity?The variation in biological factors affecting a species.The variation of genes within a species.The variation between the males and females of a species.The variation of ways in which genetic information is passed to offspring. Two objects, objects X and object Y are heldtogether by a string light and are released from restnear a planets surface in the orientation that isshown in the figure. Object X has a greater massthan object Y. A graph of the acceleration as afunction of time for the systems center of mass isshown for the 4s. The positive direction is.considered to be upwards. How does the speed ofobject X Vx Compare to that of the systems speed Vsafter the objects have fallen for 4s? 3. An aeration tank has the volume of 200 m^3 with 4 m depth. The experiment is conducted at 20C for tap water. The oxygen transfer rate is 18.7 kg/hr. (1) Determine the oxygen solubility at 20C and 1 atm (oxygen in air is 21% ), (2) Determine the mean hydrostatic pressure on the rising air bubbles, (3) Determine the overall volumetric mass transfer coefficient in the liquid phase. Burgers cost $4 each and fries cost $2 each.Write an equation that represents the total cost, 'T', of 'B', burgers and 'F', fries. Select the correct answer.A group of 6 students is surveying their classmates to get feedback about the different clubs theyre involved in. If each student surveys more than 2 of their classmates, the total number of completed surveys, , is given by the equation .Which could be the domain of this function?A.B.C.D. need help asap please!! Jaylynn has a spinner with 4 sections she spins the spinner 100 times and records the experimental probabilities for each outcome describe a spinner for which the theoretical probability of spinning each outcome is similar to the experimental probability I WILL MARK BRAINLIEST! Over and over again I have found myself asking: "Who worships here? Who is their God? Where were their voices when the lips of Governor Barnett dripped with words of interposition and nullification? Where were their voices of support when tired, bruised, and weary Negro men and women decided to rise from the dark dungeons of complacency to the bright hills of creative protest?"Type of figurative language: Meaning of figurative language:Effect on tone and mood:Effect on audience: