how recombinant dna is made and manipulated
A recombinant DNA is made and manipulated by incorporating gene of interest with the help of restriction endonucleases and DNA ligases.
A recombinant DNA can be defined as the DNA made in laboratories by joining the desired DNA strands of two organisms. The process of formation of recombinant DNA is known as recombinant DNA technology.
To make recombinant DNA, restriction endonucleases and DNA ligases are an important prerequisite.
Restriction endonucleases are also known as molecular scissors and are used to cut the DNA strand at the specific desired sites where the DNA strand containing the desired gene of interest has to be inserted.DNA ligases are also known as molecular glue as they function in sticking or joining together the two DNA strands.The desired gene of interest is isolated from the organism with the help of a specific restriction endonuclease and transferred into the host with the help of a vector such as plasmid. This gene is inserted into the plasmid and then this plasmid is allowed to enter the host through various methods such as electroporation, gene gun method, etc.
This plasmid then get integrated with the host genetic material (DNA) where it multiplies with it and undergo transcription and translation processes, in turn producing the desired results. This is how recombinant DNA is made and manipulated.
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the _ of a neuron receives inputs while the _outputs neural impulses.
The dendrites of a neuron receive inputs in the form of chemical or electrical signals from other neurons or sensory receptors. These signals are then integrated in the cell body or soma of the neuron.
The axon of the neuron then outputs neural impulses, called action potentials, which travel down the length of the axon and are transmitted to other neurons or target cells. The process of receiving inputs and integrating them in the cell body is known as synaptic integration. The cell body contains the nucleus of the neuron and is responsible for maintaining the metabolic and biosynthetic functions of the neuron. The axon is a long, thin, cylindrical structure that extends from the cell body and is responsible for transmitting neural impulses to other neurons or target cells.
The dendrites and cell body together are referred to as the receptive region of the neuron, while the axon and its terminal branches are referred to as the output region of the neuron. The point at which the axon of one neuron communicates with the dendrites or cell body of another neuron is known as a synapse.
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true or false alveolar ducts consist of cartilage rings, smooth muscles, and endothelium.
The statement, "Alveolar ducts consist of cartilage rings, smooth muscles, and endothelium" is FALSE.
Alveolar ducts are the small ducts that connect the respiratory bronchioles and the alveolar sacs in the lungs. Alveolar ducts are essential parts of the respiratory system that helps in the oxygenation of blood. Alveolar ducts are lined with simple squamous epithelium, which is a single layer of flattened cells.
The squamous cells lining the alveolar ducts help in the exchange of gases between the lungs and the bloodstream.Therefore, the correct answer to the given question is:FALSE. Alveolar ducts do not consist of cartilage rings or smooth muscles, but they are lined with simple squamous epithelium.
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if the p53 gene was inactivated, what would the likely impact be on the cell? group of answer choices all of these dna damage could accumulate the g1/s checkpoint would not function cdk activity would not be appropriately regulated
The cell may suffer catastrophic effects from the inactivation of the p53 gene, such as DNA damage buildup, G1/S checkpoint malfunction, and deregulation of CDK activity.
If the p53 gene was inactivated, it would likely impact the cell in the following ways:
DNA damage might build up, but the tumour suppressor gene p53 works to stop cells from accumulating DNA damage. Cells may build up DNA damage if p53 is deactivated, which can result in mutations and possibly cancer.Without p53, the G1/S checkpoint would not be functional, which would compromise the cell cycle's regulation. Before the cell enters the S phase of the cell cycle, this checkpoint makes sure that the DNA has been properly duplicated. The G1/S checkpoint may not operate correctly if p53 is inactive, which could result in cells entering the S phase with DNA damage or other problems.As p53 controls the activity of CDKs, which are enzymes that control the cell cycle's advancement, CDK activity would not be properly regulated. If p53 is deactivated, CDK activity might not be properly controlled, which might result in unchecked cell growth and perhaps cancer.To know more about p53 ,
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Whcih of the following scenarios would result in a person's inability to keep a memory? a in the hippocampus; inability to produce enough EPSPs to drive Mg out of the cell, therefore Ca channels responsible for increased postsynaptic sensitivity remained blocked O b. in the hippocampus; decreased glutamate release from presynaptic neurons repolarize the cell, resulting in increased AMPA receptors necessary for increased Na into the cell OCIn the hippocampus, increase in glutamate release from presynaptic neurons causes excitation of the second messenger pathway system responsible for blocking Me keeping it in the cell, and causing the cell to hyperpolarize O d. In the hypothalamus, decreased glutamate release from presynaptic neurons repolarize the cell, resulting in increased AMPA receptors necessary for increased Na into the cell e. In the hypothalamus; inability to produce enough EPSPs to drive Mg out of the cell, therefore Ca channels responsible for increased postsynaptic sensitivity remained blocked For this question. Assume a hypothetical postsynaptic neuron has three presynaptic inputs: neurons A T. and M. Also, assume neuron Mis inhibitory. If neurotransmitter from A causes a slight depolarization of the postsynaptic cell, then the a. synapse is excitatory b. synapse is inhibitory O c synapse could be excitatory or inhibitory O d. postsynaptic cells membrane potential has decreased e. a and d
The scenario that would result in a person's inability to keep a memory is option A: In the hippocampus, inability to produce enough EPSPs to drive Mg out of the cell, therefore Ca channels responsible for increased postsynaptic sensitivity remained blocked.
In this scenario, the insufficient generation of excitatory postsynaptic potentials (EPSPs) prevents the removal of magnesium (Mg) from the cell.
As a result, the calcium (Ca) channels responsible for increasing postsynaptic sensitivity remain blocked. Calcium influx is crucial for various processes involved in memory formation and synaptic plasticity in the hippocampus, including long-term potentiation (LTP).
Therefore, the inability to generate enough EPSPs and unblock the Ca channels impairs the synaptic changes necessary for memory retention.
Therefore, the correct option is A, In the hippocampus, inability to produce enough EPSPs to drive Mg out of the cell, therefore Ca channels responsible for increased postsynaptic sensitivity remained blocked.
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What is produced when depolarization of a cell membrane reaches threshold?
When depolarization of a cell membrane reaches the threshold, an action potential is produced. Here's a step-by-step explanation:
1. Depolarization occurs when positive ions, such as sodium (Na+), enter the cell, causing a change in the cell membrane's electrical potential.
2. As the cell membrane depolarizes, it moves towards a less negative or even positive electrical potential.
3. The threshold is a specific level of depolarization needed to trigger an action potential.
4. When depolarization reaches the threshold, voltage-gated ion channels open, allowing more ions to flow across the cell membrane.
5. This rapid exchange of ions causes a spike in the electrical potential, producing an action potential.
6. The action potential propagates along the cell membrane, ultimately leading to the transmission of signals in neurons or the contraction of muscle cells.
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what is the advantage of plasmolysis to the terrestrial plants?
Answer:
plasmolysis make the plants floopy, when water passes out through osmosis the plant become limb
How do fish use diffusion in respiration?
Answer: you first give the mouth to mouth
Explanation: then you do chest compressions
Answer:
Fish and other aquatic organisms use gills to take up oxygen dissolved in the water and diffuse carbon dioxide out of the bloodstream. Some insects utilize a tracheal system that transports oxygen from the external environment through openings called spiracles.
Explanation:
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Which term is used to describe the continued divergence of species based on the low fitness of hybrid offspring?
a. reinforcement
b. fusion
c. stability
d. punctuated equilibrium
Reinforcement is the term that describes the continued divergence of species based on the low fitness of hybrid offspring.
Reinforcement is the process of speciation. Here, natural selection results in the reproductive isolation of the two population of species. This isolation can be complete or incomplete. If incomplete, mating can occurs between the two populations and the resultant hybrid has low fitness.
Divergence refers to the separation of two populations of homologous nature due to some reason like speciation, migration, etc. to form different species. These different species although have a common ancestor. Such species have same anatomical structures but perform different function due to adaptations.
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What is inversion?
When the gene of interest inserts into the plasmid without DNA ligase joining the ends
the gene of interest inserts into the plasmid backwards
the plasmid closes up and is joined back together without the gene of interest
the gene of interest inserts into the plasmid upside down
An inversion is a type of genetic mutation that occurs when a segment of DNA is reversed or flipped in orientation. It can occur spontaneously or can be induced artificially in the laboratory using techniques such as DNA inversion. d) The gene of interest inserts into the plasmid upside down.
An inversion is a genetic mutation that involves the reversal or flipping of a segment of DNA within a chromosome. This mutation can occur spontaneously due to errors in DNA replication or repair, or it can be induced artificially in the laboratory through genetic engineering techniques. Inversions can have a variety of effects on an organism, depending on the location and orientation of the inverted segment. In some cases, inversions can disrupt important genes or regulatory regions, leading to developmental abnormalities or disease. In other cases, inversions can create new genetic variation that may be beneficial or adaptive in certain environments. Understanding the mechanisms and effects of inversions is important in fields such as genetics, evolution, and biotechnology.
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Some birds follow moving swarms of army ants in the tropics. As the ants march along the forest floor hunting insects and small vertebrates, birds follow and pick off any insects or small vertebrates that fly or jump out of the way of the ants. What is the result of the association between birds and ants?.
Some birds follow transferring swarms of military ants in the tropics. because the ants march alongside the forest ground hunting bugs and small vertebrates birds observe and pick off any bugs or small vertebrates that fly or leap out of the way of the ants is commensalism.
Ant-following birds benefit from the relationship by way of staying just in advance of the ants and taking pictures of prey animals that can be disturbed by the ants. even as early studies advised that the birds foraging may in turn advantage the ants, it's miles possible that the birds do away with prey that the ants would capture.
Ant followers are birds that feed by following swarms of military ants and take prey flushed by way of the one's ants. The pleasant-acknowledged ant-fans are 18 species of antbird inside the circle of relatives Thamnophilidae, however other families of birds may additionally observe ants, such as thrushes, chats, ant-tanagers, cuckoos, motmots, and woodcreepers.
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explain how the last food chain represents a full circle of life
Answer:
the last food chain is the last predator of the enature food chain which ends that particular food chain and inturn the circle of life
The last food chain represents a full circle of life by:-
Detritivores and decomposers are the final part of food chains. Detritivores are organisms that eat dead plant and animal remains. Energy is passed from plant to animal to animal to decomposer and back to plant.What is food chain?The food chain describes who eats whom in the wild. Every living thing needs food to survive.Each food chain is a possible pathway that energy and nutrients can follow through the ecosystem.To know more about food chain here
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If the snake were removed from the ecosystem, which organism would immediately be impacted? (6b)
A.
The mouse population would increase because one of their predators was removed.
B.
The rabbit population would increase because one of their predators was removed.
C.
The mouse population would increase because they have less competition.
D.
The rabbit population would increase because they have less competition.
Answer:
A or B
Explanation:
What ever is right under the snake in the food chain.
in a certain breed of plants, dark green is determined by the dominant allele 'g' and light green is determined by the recessive allele 'g'. all gg plants are dark green. however, in heterozygous gg plants penetrance of the dark green trait is only 75%.the parental cross gg x gg was made and dark green f1 offspring were intercrossed, and 400 f2 offspring resulted. how many dark green f2 plants would be seen? answer
If the parental cross GG x gg was made and dark green F1 offspring were intercrossed, and 400 F2 offspring resulted, the dark green F2 plants would be seen 300.
From the description, we are given:
Dark green = dominant allele GLight green = recessive allele gWhen we cross GG аnd gg, 100% of the offspring will be heterozygous. If the heterozygotes show 75% penetrаnce meаning thаt there is а 75% probаbility thаt the plаnt thаt hаs the G gene will аctuаlly show it in it's phenotype.
So thаt meаns thаt 75% of the offspring should hаve dаrk green color, which meаns thаt 300 plаnts will hаve the expected phenotype аnd 100 will not.
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T/F In general, there are two types of AEDs: fully automated and semiautomated. Semiautomated AEDs: Indicate to the operator when a shock is advised so he or she can push another button to deliver the shock.
"In general, there are two types of AEDs: fully automated and semiautomated. Semiautomated AEDs: Indicate to the operator when a shock is advised so he or she can push another button to deliver the shock" is true.
Automated External Defibrillators (AEDs) are portable electronic devices used to treat sudden cardiac arrest (SCA). They analyze the heart's rhythm and, if necessary, deliver an electric shock to restore normal heart rhythm.
In general, there are two types of AEDs: fully automated and semiautomated.
Fully Automated AEDs: These devices are designed to automatically analyze the heart's rhythm and deliver a shock if needed. The operator does not need to manually initiate the shock delivery. The AED detects the need for a shock and, after a brief countdown or voice prompt, delivers the shock automatically. This type of AED is often used in public places or locations where individuals may have minimal or no training in using an AED.Semiautomated AEDs: These devices also analyze the heart's rhythm, but they require the operator to manually deliver the shock. When a semiautomated AED determines that a shock is necessary, it will indicate to the operator through visual or auditory prompts. The operator then needs to press a button to deliver the shock. This additional step ensures that the operator is actively involved in the decision to deliver the shock. Semiautomated AEDs are commonly used in various settings, including healthcare facilities, where trained individuals are available to operate the device.Both fully automated and semiautomated AEDs are designed to assist in the prompt delivery of defibrillation to individuals experiencing SCA. They provide clear instructions and guidance to users, enabling even those without extensive medical training to respond effectively in emergency situations.
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Age-related changes associated with the cardiac system include which conditions? select all that apply.
Age-related changes associated with the cardiac system includes the following conditions: (c) Endocardial fibrosis, and (d) Increased size of the left atrium.
Cardiac system is actually the cardiovascular system that functions to circulate blood to the whole body. It regulates the circulation of oxygenated blood from heart to the whole body and also the deoxygenated blood from the heart to the lungs.
Endocardial fibrosis is an endemic disease. In this disease thee occurs fibrosis in the apical endocardium of right ventricle, left ventricle, or both. A certain specific cause for the disease has not been identified. The disease can be caused due to infection from parasites, protozoans or due to inflammation.
The question is incomplete, the complete question is:
Age-related changes associated with the cardiac system include which conditions? Select all that apply.
Increase in the number of SA node cellsMyocardial thinningEndocardial fibrosisIncreased size of the left atriumTo know more about cardiac system here
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Which factors affect the type of cloud that will be formed? select three choices. altitude temperature color of clouds size of ice pellets whether water condenses as liquid or ice
Answer:
To conclude, many factors affect cloud formation, including topography, air temperature, and humidity.
Explanation:
The factors affect the type of cloud that will be formed are temperature , color of clouds and water condenses as liquid or ice.
These factors affect the formation of clouds as cloud formation will have the all factors like humidity, water, temp, and also sometimes light.
What is altitude?It is the height of mountains or any plains, pleautue, and several other earth mountains, valley and sometimes also of the himalyan mountains.
Thus it is well explained.
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May you please be able to help me on this
1. The male superhero cannot teleport. So, the probable genotype is Xt Y
While the female superhero can teleport, but her mother could not. The probable genotype would be XTXt.
In order to get these genotypes for teleporting abilities, we first assign thegenotypes of the parents, which are:
Father- XT Y
Mother (do not have teleporting abilities)- XtXt
Which of the following should be used to make a precise measurement of liquid volume
Answer:
one of these, beaker, test tube,or graduated cylinder.
Explanation:
they help measure the amount of liquid in close measures.
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HELP! I'll give the brainliest!!
Answer:
In facilitated diffusion, molecules move down their concentration gradient.Explanation:
In facilitated diffusion, substances move into or out of cells down their concentration gradient through protein channels in the cell membrane. Simple diffusion and facilitated diffusion are similar in that both involve movement down the concentration gradient.
Osmosis refers specifically to the movement of water across a semipermeable membrane, with the solvent (water, for example) moving from an area of low solute (dissolved material) concentration to an area of high solute concentration.
Active transport is the movement of dissolved molecules into or out of a cell through the cell membrane, from a region of lower concentration to a region of higher concentration. The particles move against the concentration gradient , using energy released during respiration .
ASAP
What does the ‘fluid’ part of the Fluid Mosaic Model mean?
A. The proteins float around in the membrane as if in a fluid.
B. The lipids are suspended in a fluid.
C. The membrane creates necessary fluids for the cell.
D. It is fluid because it is adaptable to change.
Answer:
A) The proteins float
Explanation:
The proteins float around freely
2. Identify the tectonic feature created at location B (south america)
Answer:
Andes mountains
Explanation:
Name the hormones and enzymes in the human stomah
Answer:
Pepsin is the main gastric enzyme. It is only produced by the stomach cells which people call 'chief cells' in its inactive form pepsinogen, which is a zymogen. Pepsinogen is then activated by the stomach acid and into its active form which is known as pepsin.
The types of hormones in the human stomah is Gastrin and cholecystokinin.
4. Which system helps keep you healthy by carrying white blood cells throughout
the body which trigger immune responses when needed?
O A. Digestive system
B. Endocrine system
C. Respiratory system
D. Lymphatic system
5. True or False: The excretory system is responsible for inhaling and exhaling,
Answer:
4. It is the lymphatic system
5. False, the excretory system is responsible for waste material.
Explanation:
how much is the tilt of earths axis?
Answer:
about 23.5 degrees
Explanation:
Answer:
It's about 23.5 degrees
Explanation:
Earth's axial tilt (also known as the obliquity of the ecliptic) is about 23.5 degrees. Due to this axial tilt, the sun shines on different latitudes at different angles throughout the year. This causes the seasons. Uranus has the largest axial tilt in the solar system.
Based on the DNA sequence below, which of the species is most closely related to the unknown species?  Species 1: GTT/CCA/GAA/AAT/CCT Unknown: AAT/CCT/GAA/AAT/CCA. Species 2: ATA/CCT/GTT/AAT/GGA. O Species 1. O Species 2
Based on the DNA sequence, the species that is most closely related to the unknown species is species 1, as it has more homologies than species 2. A bigger distance can be traduced in a higher number of mutations or modifications in the DNA sequence.
The correct answer is: Species 1.
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What claim or claims can you make about the impact of climate change on sea level rise?
What is your evidence for this claim?
Answer:
POLUTION
Explanation:
Answer:
I can make the claim that climate change and the sea level rise have a positive correlation. The graph shows that as the impact of climate change increases so does the sea level conveying that they are positively correlated.
An organism with a cell wall would most likely be unable to take in materials through?
Answer:An organism with a cell wall would most likely be unable to take in materials like food via phagocytosis because their cell wall is inhibiting that change in cellular structure.
Two tubes A & B were set up as shown above. Both tubes were left indoors for 50 minutes and then taken outdoors for another 50 minutes. The tubes were weighed every 10 minutes. The table shows the results obtained.
Answer:
Explanation:
Question A
Evaporation happens because fast moving molecules break out of the surface of the water. The fact that there is part of a plant in the water means that the water travels up to the leaves where it evaporates. There are two sources of evaporation in test tube A and only one in B.
Question B
The Cotton plug in the top of the test tubes slows the process down. There are still molecules that are escaping but the cotton absorbs them especially in test tube B. The molecules are free to go in test tube A. They make their way to the leaves where they evaporate.
Part C
You'll have to do the rest of this yourself. The graph for Test Tube A should be less (closer to the x axis) than B.
The rest of the answers are explained in A and B
Which of the following is an acquired trait in humans?
Eye color
Hair color
Blood type
Table manners