The apparatus used to purify the PCR product is a microconcentrator column. PCR or Polymerase chain reaction is a lab technique that amplifies a single copy or a few copies of DNA sequences.
It helps to create a large amount of specific DNA fragments from small or trace amounts of DNA. There are several steps involved in PCR, and purification is one of the crucial steps in the PCR process.The PCR product contains the desired DNA fragment and other substances such as primers, dNTPs, buffer, etc.
It is essential to remove these substances as they can interfere with the downstream applications. Therefore, the PCR product needs to be purified before proceeding to the next steps of DNA sequencing, cloning, or other applications.The purification of PCR products can be done by different methods such as gel extraction, ethanol precipitation, spin column purification, or microconcentrator column.
Microconcentrator column or spin column purification is a simple and quick method to purify the PCR product. It involves binding the PCR product to a microconcentrator column and then washing away the contaminants using a buffer solution. Finally, the pure PCR product is eluted from the column using an elution buffer. Thus, the microconcentrator column is the apparatus used to purify the PCR product.
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a species of crab produces 1,000's of eggs at a time. these crabs will have a high mortality rate when weather patterns are severe. this crab species is an example of a _______.
A. selected species
B. ecosystem
C. a small population
The crab species described, which produces a large number of eggs but experiences high mortality rates during severe weather patterns, is an example of a small population. Option C is correct.
A small population refers to a relatively low number of individuals within a species that occupy a particular geographic area. In this case, despite the high reproductive output of the crab species (producing thousands of eggs), the mortality rate during severe weather patterns suggests that the population size remains small due to the high levels of mortality.
Severe weather patterns can negatively impact the survival and reproductive success of individuals within a population. If the mortality rate is high during such events, it can lead to a reduction in the population size. In this scenario, even though the species produces a large number of eggs, the overall population remains small due to the high mortality rate caused by severe weather conditions.
Hence, C. is the correct option.
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why are coal, oil, and natural gas called fossil fuels? view available hint(s)for part a why are coal, oil, and natural gas called fossil fuels? all of these energy sources contain organic material from land animals. all of these resources consist of material taken from various animal and plant fossils that have been impacted over the years. there are no fossils involved in the creation of these resources; they are nicknamed fossil fuels because of the millions of years required for their formation. coal is made from fossil plant matter, and oil and natural gas are made from fossil plant matter and fossil marine animal matter. all of these energy sources are derived forms of coal, which is the fossil of plant material.
Coal, oil, and natural gas are called fossil fuels because they are formed from the fossilized remains of ancient plants and animals
Over millions of years, the organic material from these fossils has been subjected to heat and pressure, resulting in the formation of coal, oil, and natural gas.
Coal is primarily made from fossilized plant matter, which was buried in swamps and subjected to heat and pressure over millions of years. Oil and natural gas are formed from a combination of fossilized plant and marine animal matter, which accumulated on the ocean floor and were buried under sediment.
The term "fossil" in fossil fuels refers to the fact that the organic material used to produce these fuels comes from ancient living organisms, which have been preserved in the earth's crust over millions of years. While no fossils are involved in the actual formation of these fuels, the term "fossil" is used to reflect the immense time period required for their creation.
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Bacteria with the ability to break down certain types of plastic are placed with a colony of E. coli bacteria that lack this ability. Plasmid transfer occurs between the two colonies of bacteria and some of the E. coli gain the ability to break down plastic as well. What inference can be drawn about the nature of plasmids from this observation?
A.
Plasmids are nucleic acids which can pass on traits.
B.
Plasmids are carbohydrates which provide structural support.
C.
Plasmids are proteins which build tissues.
D.
Plasmids are lipids which provide energy for the cell.
Answer:
A. Plasmids are nucleic acids which can pass on traits.
Explanation:
A plasmid is a small circular DNA molecule found in bacteria and some other microscopic organisms.
in the fick's equation below, if the membrane of a capillary becomes thickened while all other variables are constant, how would the rate of diffusion of o2 from the capillary to a tissue cell be influenced? r
A thicker membrane would negatively impact the rate of diffusion of O2 from the capillary to a tissue cell, making it harder for the cell to receive the necessary oxygen for metabolic processes.
Fick's equation describes the rate of diffusion of a substance across a membrane, such as from a capillary to a tissue cell. The equation is given as:
J = -D(dC/dx)
where J is the flux of the substance, D is the diffusion coefficient, and dC/dx is the concentration gradient across the membrane.
If the membrane of a capillary becomes thickened while all other variables remain constant, the rate of diffusion of O2 from the capillary to a tissue cell would be reduced. This is because the diffusion coefficient, D, would be decreased due to the thicker membrane. A thicker membrane would create more resistance to the movement of O2 molecules, leading to a decrease in the overall rate of diffusion.
Additionally, the concentration gradient across the membrane (dC/dx) may also be affected. A thicker membrane may lead to a smaller concentration gradient, as there would be less of a difference in O2 concentration between the capillary and tissue cell.
Overall, a thicker membrane would negatively impact the rate of diffusion of O2 from the capillary to a tissue cell, making it harder for the cell to receive the necessary oxygen for metabolic processes.
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How would the presence of an extra solute - glucose – in the urine affect the urine is osmotic pressure and tendency to draw water by osmosis?
When the extra amount of glucose is present then the reabsorbtion capacity of kidney glucose remains in the filtrate , which is causes an increase in osmotic pressure causing potassium and water to move out into the urine.
Hyper-tonic additional glucose stimulates osmotic fluid that increase plasma and blood volumes. Glucose provides nutritional aspects and also used as a metabolic testing. Decreased intravascular osmotic pressure from decreased concentration of plasma protein , particularly.
Osmotic pressure is required to prevent the net movement of water into solute of the solution when the solution are separated by a selectively permeable membrane.Serum albumin protein found in blood plasma that helps in maintain osmotic pressure between blood vessel and tissues.
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5. What are virus hoaxes? Why are the hoaxes sometimes more dangerous than an actual virus?
Answer:
An actual computer virus is a malicious software, often known as malware, that can harm a computer and its users.
Virus hoaxes are false or misleading information about viruses that circulate through various communication channels.
They can be more dangerous than actual viruses due to their ability to spread quickly, cause panic, and undermine effective public health measures.
Virus hoaxes are deceptive messages or claims that often exaggerate the severity or impact of a particular virus. They can be spread through social media, email chains, or word of mouth. These hoaxes may include misinformation about symptoms, transmission methods, or false remedies, leading people to take ineffective or even harmful actions.
What makes virus hoaxes particularly dangerous is their potential to create panic and misinformation at a rapid pace. The viral nature of social media and other communication platforms allows these hoaxes to reach a wide audience within a short period. As a result, people may make decisions based on false information, such as avoiding necessary medical treatment, taking unnecessary precautions, or spreading fear and misinformation to others.
Moreover, virus hoaxes can undermine public health efforts by diverting attention and resources from legitimate preventive measures. They can erode trust in healthcare authorities and disrupt the dissemination of accurate information, making it harder for individuals to make informed decisions and follow recommended guidelines.
This can have severe consequences, especially during outbreaks or pandemics, where timely and accurate information is crucial for public safety. Therefore, it is essential to verify the credibility of information and rely on trusted sources to mitigate the risks associated with virus hoaxes.
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Biodiversity in a particular ecosystem
A. is the total number of species in that ecosystem
B. includes the variability of the individual genes
C. impacts the function of the ecosystem in which the species live
D. All of the choices are correct.
Biodiversity in a particular ecosystem : D.) All of the choices are correct. The term biodiversity refers to the degree of variability of living organisms in a particular ecosystem. Hence, option D) is the correct answer.
It includes the total number of species in the ecosystem and the variability of the individual genes, as well as the influence of the ecosystem on the species. The function of the ecosystem, as well as the species that inhabit it, are affected by biodiversity.
The more diverse an ecosystem is, the more resilient it is to environmental changes and disturbances. It also plays a crucial role in the ecosystem's functioning by influencing nutrient cycling, soil formation, and water purification.
Therefore, the correct answer is option D, that all of the choices are correct. Biodiversity is a term used to describe the variety of living things in a particular ecosystem. It refers to the range of species in a region and the genetic variation that exists within and between them. The complexity of an ecosystem is determined by its biodiversity.
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Please help me I’m begging
D. The desert wildflowers recieve energy from Sun to make their own food
E. The millipedes return nutrients from decomposing organisms back into the soil.
The graph shows changes to a population over time. Four points on the graph are
identified by number. Which number indicates that the population is growing at its
fastest rate?
Answer:
Explanation:
2 because it's going upward.
What is the process of passing genes?
Transcription and translation are the two main procedures. Gene expression is the result of transcription and translation working together.
The information contained in a gene's DNA is transferred to an analogous molecule called RNA (ribonucleic acid) in the cell nucleus during transcription. However, when people discuss the passing on of genes, they typically do not refer to the passing on of genes during cell division from one cell to another.
Instead, they typically refer to genes that are passed on from parents to their offspring. Heredity or "inherited genes" refer to this. An egg from the mother and sperm from the father combine to make a fetus. Each chromosome in the egg and sperm makes up one half of the complete set. The embryo is created from the sperm and egg.
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what are the main hormones in the female reproductive system? check all that apply. a. estrogen b. progesteronec. testosterone
The main hormones in the female reproductive system that are involved in this system are estrogen, progesterone, and testosterone.
Estrogen is the primary female sex hormone and is responsible for the development and maintenance of the female reproductive system and secondary sex characteristics. It is also responsible for the regulation of the menstrual cycle, the release of eggs from the ovaries, and the development of breasts and other body parts.
Progesterone is another important hormone in the female reproductive system. It is produced by the ovaries and helps to regulate the menstrual cycle by preparing the uterus for implantation of a fertilized egg. It also helps to maintain the lining of the uterus and is involved in the development of the placenta during pregnancy.
Testosterone is a hormone found in both men and women. In women, it is produced in small amounts by the ovaries. It plays a role in libido and helps to regulate the development of secondary sex characteristics. It is also involved in the regulation of the menstrual cycle and the production of eggs from the ovaries.
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Under anaerobic conditions, such as intense exercise, pyruvate is converted to ____________ . This 3-carbon molecule travels through the blood to the liver, where it can be converted to glucose by the ____________ cycle.
Under aerobic conditions, pyruvate enters the mitochondrion, where it is converted to ____________ . This 2-carbon molecule goes on to participate in a series of oxidative reactions called the ____________ cycle.
Options:
acetyl CoA
citric acid
ATP
lactate
glucose
Cori
Answer:
Under anaerobic conditions, such as intense exercise, pyruvate is converted to lactate. This 3-carbon molecule travels through the blood to the liver, where it can be converted to glucose by the cori cycle.
Under aerobic conditions, pyruvate enters the mitochondrion, where it is converted to acetyle CoA . This 2-carbon molecule goes on to participate in a series of oxidative reactions called the Citric acid cycle.
Explanation:
In the question ,among the given 6 options ,the simplest way to find the answer can be ,to find either the option is aerobic (presence of free oxygen ) or anaerobic (lack of free oxygen). Among the options , glucose is an incorrect option as it is a carbohydrate and used in respiration process .In aerobic process ,the glucose can be break down but in anaerobic ,it can only be used.
Other option is ATP- ATP is the energy source of the cell and used for storing energy or reactions of the cell,thus this option do not belong to the given question.
thus its clear the rest four options are the answer .
among four two options belong to aerobic and the rest two are anaerobic.
Under anaerobic conditions-
The pyruvate is converted into lactate because under the absence of oxygen only the NAD+ can be regenerated, thus further can allow glycolysis.
The cori cycle is also called lactic acidic cycle, thus this cycle is a metabollic pathway produced by anaerobic glycolysis (which takes place by regeneration of NAD+)transporting the molecules from blood to liver and further converting to glucose
Under aerobic conditions,-
The pyruvate is converted into Acetyle CoA so as to further produce citric acid and then citric acidic cycle. Thus Acetyle CoA forms more ATP which help in the regeneration of NAD+ and production of citric acid cycle takes place.
HENCE , The correct options are - lactate cori acetyle CoA citric acid.
Discuss the advantages and disadvantages of using (a) an unordered file, (b) an ordered file, and (c) a static hash file with buckets and chaining. Which operations can be performed efficiently on each of these organizations, and which operations are expensive? What is the difference between the directories of extendible and dynamic hashing? Describe the mismatch between processor and disk technologies.
(a) Unordered files have efficient insertion and no need for sorting, but slow searching and inefficient deletion. (b) Ordered files offer faster searching and efficient range queries, but slow insertion and deletion. (c) Static hash files with buckets and chaining provide constant-time operations, but require additional memory and depend on the hashing function.
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help meh plz
Which abiotic factor would increase the sustainability of an ecosystem?
An increase in the available water in an ecosystem
An increase in the human interference in the ecosystem
A decrease in the amount of sunlight in the area.
A decrease in the number of available producers in the area.
An increase in the available water in an ecosystem would increase the sustainability of an ecosystem.
An increase of human interference can negatively impact local flora and fauna populations.
A decrease in the amount of sunlight would negative impact plants.
A decrease in the number of available producers will negatively impact the entire food chain/food web.
Answer:
An increase in the available water in an ecosystem.
Explanation:
An abiotic factor of an ecosystem is a non-living factor.
"A decrease in the number of available producers in the area. " Would be a biotic factor and would affect the environment for the worse. It would be the same with the second option too.
The third one is an abiotic factor but it would affect the environment for the worse.
The best answer would be the first option.
Hope this helps.
UNIT 3: Force, Motion, Energy
Lesson 1: Speed, Velocity, and Acceleration
RM 12
Distance vs. Time
50
40
B
E
30
Distance (m)
20
10
с
D
2
4.
6
8
10
Time (sec)
1. Between which two points does the object stop?C
Answer:
its d
Explanation:since the number is low
help please 30 points and brainliest for correct answer :(
Which of the following is a future consequence of the use of hydropower from dams on the environment?
It generates no greenhouse gases.
It can be used in isolated locations.
A dam and reservoir can flood habitats.
It produces less noise than other energy.
The dam and reservoir can flood habitats is a is a future consequence of the use of hydropower from dams on the environment .
What is hydropower?A a renewable energy source, or one that has a capacity for hydraulic use over time. Water fits as a substance that is always recomposing in the terrestrial environment.
Today it is known that they cause major environmental impacts, such as the decomposition of submerged vegetation that gives rise to gases such as methane, carbon dioxide and nitrous oxide, which cause climate change.
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PLEASE ANSWER I WILL GIVE BRAINLIEST!!! If two babies both had red tails, would their genotypes for that trait have to be identical? Show the possible genotypes.
A pink snapdragon is crossed to a white snapdragon. What is the probability of getting a red snap dragon
Answer:
0%
Explanation:
Hope this helped! ^^
How is rDNA made?Restriction enzyme action of EcoRI:
In conclusion, EcoRI is employed to cleave rDNA at its specific recognition site, resulting in pieces with complementary sticky ends that can be joined to generate recombinant DNA.
In recombinant DNA technology, EcoRI is a common restriction enzyme. The short summary of EcoRI's role in rDNA synthesis is as follows: DNA isolation: The first step is to isolate the desired DNA, which can be accomplished in a number of ways depending on the DNA's origin.
A host cell receives the recombinant DNA after which it can reproduce and make many copies of the rDNA. EcoRI addition: The isolated DNA is given EcoRI. A particular sequence of six nucleotides is recognized by the restriction enzyme EcoRI.
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If the atom had one less electron it would be a
Answer:
If it has fewer electrons than protons it has a net positive charge and is known as a cation. The ionic state may be denoted as a following superscript (e.g. O2-, Fe2+). The common ionic states of a atom are known as its valences.
Explanation:
Mollusk fossils were excavated and collected from a coastal cliff. scientists studying the fossils observed patterns of changing shell shape in the mollusks. the scientists identified a period of stability or stasis, followed by rapid change, and then stasis again with regard to shell shape. What statements best predicts the result of a comparison between the fossils found before and after the period of rapid change?
Mollusk fossils were excavated and collected from a coastal cliff. scientists studying the fossils observed patterns of changing shell shapes in the mollusks. Based on the observations of the scientists studying the mollusk fossils, it can be predicted that there would be a noticeable difference in the shell shape of the mollusks found before and after the period of rapid change.
What is the best prediction for the comparison between the mollusk fossils found before and after?
The best prediction for the comparison between the mollusk fossils found before and after the period of rapid change is that the shell shapes will be significantly different. The rapid change indicates a distinct shift in the characteristics of the shells, while the stasis periods before and after suggest that the mollusks were relatively stable and not undergoing significant changes in shell shape during those times.
The fossils found before the period of rapid change would likely show a more stable and consistent shell shape, while the fossils found after the period of rapid change would show a greater diversity in shell shapes as a result of the rapid evolutionary changes that occurred during that period.
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In the example in the animation, uracil is incorrectly paired with guanine. What is the effect on the dna sequence if base excision repair does not remove the uracil and dna replication is allowed to proceed?
Mutation will occur in the DNA sequence if base excision repair does not remove the uracil and DNA replication is allowed to proceed.
What occurs when DNA damage is not repaired prior to replication?DNA's stability and integrity are crucial to life since it houses each live cell's genetic information. However, DNA is not inert; rather, it is a chemical structure that can be harmed by the environment. If the damage is not repaired, it can result in mutation and even disease.
What occurs if nucleotide excision repair is unsuccessful?Base excision repair (BER) is in charge of repairing broken DNA bases, including those left over after oxidative damage, deamination, and SSBs. Point mutations may occur as a result of these lesions not being repaired.
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Many types of plants produce fruit which is eaten by animals. The seeds of these plants are typically undigestible. Which of the following is an advantage for plants having undigestible seeds? It allows seeds to reside in the gut until conditions are appropriate for germination. It ensures that flowers of these plants will be pollinated. It allows the seeds to be dispersed into other areas. It provides a source of nutrients for the germinating seed.
The advantage for plants having undigestible seeds is that it allows seeds to reside in the gut until conditions are appropriate for germination.
Fruits are a source of nutrition for animals that consume them, and they are also a means of seed distribution for plants that produce them. Many types of plants generate fruit that is consumed by animals; nevertheless, the seeds of such plants are usually indigestible. As a result, the seeds can be dispersed into new areas by animals that eat the fruit.
However, having undigestible seeds provides a distinct benefit to plants: it allows seeds to reside in the gut until conditions are appropriate for germination, which aids in seed growth and germination.
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this answer i need pls ill mark brainliest
All - growth, exchange of gases, excretion, reproduction, and death (option d).
All living organisms undergo various life processes to maintain their existence. Let's analyze each option to determine which life processes are carried out by an organism's cells:
A. Only growth and exchange of gases: While cells are involved in growth and exchange of gases, they also participate in other life processes. This option is incomplete.
B. Only growth, exchange of gases, and reproduction: Cells play a crucial role in reproduction as they are responsible for the production of gametes and the process of cell division. However, there are additional life processes that cells also undertake.
C. Only growth, exchange of gases, excretion, and reproduction: This option includes excretion in addition to growth, exchange of gases, and reproduction. Cells participate in excretion by eliminating waste materials. However, there is one more life process that cells experience.
D. All - growth, exchange of gases, excretion, reproduction, and death: This option encompasses all the mentioned life processes. Cells are involved in growth as they undergo cell division and increase in number. They exchange gases through processes like respiration. Cells excrete waste products. They participate in reproduction through the formation of gametes and cell division. Lastly, cells also experience death as they have a limited lifespan.
Therefore, the correct answer is D. All - growth, exchange of gases, excretion, reproduction, and death.
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which of the following correctly describes the relationship between DNA qnd differentiation if a cell
Explanation:
The relationship between DNA and differentiation of a cell is that DNA is the blueprint that instructs a cell to differentiate into a specific type of cell. Differentiation is the process of a cell changing from its original state to become a more specialized cell type. During differentiation, DNA is read and the instructions it contains are used to create new proteins and modify existing proteins that are necessary for the cell to develop into its specialized form. DNA also contains instructions for controlling gene expression, which helps to direct the cell to differentiate into a specific cell type. Differentiation is a necessary process for a cell to take on a specialized role in the body, and DNA plays an important role in controlling the process.
Answer: which of the following correctly describes the relationship between DNA and differentiation if a cell
Which of these converts light energy into chemical energy?
O the Calvin cycle
O light-dependent reactions
O light-independent reactions
O chlorophyll
Answer:
Light-dependent reactions
Explanation:
First of all, the light-independent reactions and the same thing as the Calvin cycle, so your teacher wouldn't put them in the same question if one of them was correct. Aside from that, even if you have no idea what these things are we can use the names of them to see which one light is involved in. Aha! It's the light-dependent reactions because they're dependent on light (and water) to make the chemical energy (NADPH and ATP) to pass on to the next stage, the light-independent reactions.
1- What are Biometrics? And
Why Biometrics been used in Security industry?
2- There are two types of
enrollments in Biometrics. Mention them with explanation.
3- What is Multibiometric
1. Biometrics refers to the measurement and analysis of unique physical or behavioral characteristics of individuals, used in the security industry for accurate and reliable identification. 2. There are two types of enrollments: unimodal and multimodal. 3. Multibiometric systems utilize multiple biometric characteristics to enhance accuracy and overcome limitations of individual traits.
Biometrics refers to the measurement and analysis of unique physical or behavioral characteristics of individuals. It involves the use of advanced technologies to capture and authenticate these characteristics, such as fingerprints, iris patterns, facial features, voiceprints, or even behavioral traits like gait or typing patterns.
Biometrics is used in the security industry because it offers a high level of accuracy and reliability in verifying the identity of individuals. Compared to traditional methods like passwords or ID cards, biometrics provide a more secure and convenient way of authentication, as these characteristics are difficult to forge or replicate.
The two types of enrollments in biometrics are:
a. Unimodal Enrollment: In unimodal enrollment, a single biometric trait is used for authentication. For example, using only fingerprint or iris scan for identification. This approach is relatively simpler to implement and can be cost-effective for systems that require lower security levels or have limited resources.
b. Multimodal Enrollment: In multimodal enrollment, multiple biometric traits are combined to enhance the accuracy and reliability of identification. It involves capturing and storing data from two or more biometric sources, such as fingerprints and facial features, and using them collectively for authentication.
Multimodal enrollment provides higher security and reduces the risk of false positives or false negatives, as it combines the strengths of different biometric characteristics.
Multibiometric refers to the use of multiple biometric characteristics for identification and verification purposes. Instead of relying on a single biometric trait, multibiometric systems utilize a combination of traits such as fingerprints, iris patterns, facial features, or voiceprints to enhance accuracy and reliability.
This approach leverages the distinctiveness and uniqueness of multiple biometric traits, making it more difficult for unauthorized individuals to mimic or bypass the security measures. Multibiometric systems offer increased robustness and can overcome limitations or drawbacks associated with using a single biometric modality, such as environmental factors or individual variations.
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Which phrase used by the president was specifically chosen to convince the public to support the war?
“facts of yesterday”
“unprovoked and dastardly attack”
“have already formed their opinions”
“a state of war has existed”
The phrase used by the president was specifically chosen to convince the public to support the war is “unprovoked and dastardly attack”. Thus, option "B" is correct.
How, support your answer?President Roosevelt was in office when Pearl Harbor happened, and it was the perfect occasion that the government of the United States needed to enter a war that was clearly being won by the Germans and Japanese, they had already taken decisions prior to this to help the allies, but they couldn´t directly send troops when the bombings on Pearl Harbor took place, and since the USA had never provoked, attacked or done anything to upset Japan, they were now able to declare war on Japan an its allies.
Thus, option "B" is correct.
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Answer:
B
Explanation:
Did the assignment
A “flight or fight” response occurs when the body perceives a type of threat. The hormone known as adrenaline is released which will affect a variety of body systems. Which system needs to increase its activity in order for the muscular system to respond to the perceived threat?
A)Endocrine
B)Integumentary
C)Excretory
D)Digestive
Answer:
It is the Endocrine System.
Explanation:
The Endocrine System controls and regulates almost all the hormones in your body.
Integumentary System = Skin Tissues
Excretory System = Release of Waste out of the Body
Digestive System = Breakdown of Food
can someone send me the gizmo coral reefs 1 answer key..
Answer:
Explanation:
No but of u ask the questions I can help