why are chromogenic assays (as opposed to direct measurements at the wavelength which proteins naturally absorb light) used in the lab to measure protein concentrations?

Answers

Answer 1

Chromogenic assays are used in the lab to measure protein concentrations because they are more sensitive, specific, and convenient than direct measurements at the wavelength at which proteins naturally absorb light.

In direct measurements, it can be difficult to distinguish between different proteins that may have overlapping spectra. On the other hand, chromogenic assays involve adding a specific substrate to the protein sample and measuring the resulting color change, which can be used to quantify the amount of protein present. This method is more sensitive and specific as it allows for better discrimination between different proteins and reduces background interference.

In addition, chromogenic assays can be performed in a quicker and more automated manner than direct measurements, making them a convenient choice for many laboratories. By using chromogenic assays, the laboratory can efficiently and accurately measure protein concentrations in a range of sample types, including blood, plasma, and tissue samples.

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Related Questions

in the polymerase chain reaction (pcr), early replication errors affect a larger percentage of pcr products than late replication errors.

Answers

In the polymerase chain reaction (PCR), early replication errors do not necessarily affect a larger percentage of PCR products than late replication errors.

The replication errors in PCR can occur at any stage of the reaction and are not inherently biased towards early or late replication PCR is a laboratory technique used to amplify specific DNA sequences. It involves a series of temperature cycles that include denaturation, annealing, and extension steps. During the extension step, a DNA polymerase enzyme synthesizes new DNA strands complementary to the target sequence.

Replication errors, also known as polymerase errors or mutations, can occur during the extension step of PCR due to the inherent error rate of the DNA polymerase enzyme. These errors can result in the incorporation of incorrect nucleotides, leading to sequence variations or mutations in the amplified PCR products.

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what will the results of the testcross be if the loci assort independently?

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In a testcross, the results of the loci will assort independently if they are situated on different chromosomes. Chromosomes are long, string-like structures inside the nucleus of cells that are made of DNA. The genes (loci) are situated on chromosomes.

The location of genes on chromosomes can either be the same or different. If genes are situated on different chromosomes, then they assort independently, which means that they are inherited as separate units from one another. This can be explained by considering the experiment carried out by Mendel on inheritance. In his experiments, Mendel crossed purebred tall plants with purebred short plants and observed that all the F1 progeny were tall. He then crossed these F1 progeny with short plants. If we assume that these two genes (one for tallness and the other for shortness) are on different chromosomes, then they will assort independently in the testcross. The offspring of this cross would include some tall plants and some short plants, as they have inherited each gene separately. Therefore, the results of the testcross would be a 1:1 ratio of the two possible phenotypes if the loci assort independently.

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A patient with a known case of AIDS would likely have periodic​ ________ to assess the immune status.
A.
Western blot test
B.
erythrocyte sedimentation rate​ (ESR)
C.
CD4 cell count
D.
​enzyme-linked immunosorbent assay​ (ELISA

Answers

A patient with a known case of AIDS would likely have periodic CD4 cell count tests to assess the immune status.

CD4 cell count is an important measure of immune function and is commonly used in monitoring individuals with HIV/AIDS. CD4 cells, also known as T-helper cells, play a crucial role in coordinating the immune response. In individuals with AIDS, the HIV virus specifically targets and destroys CD4 cells, leading to a weakened immune system.

By regularly monitoring CD4 cell counts, healthcare providers can evaluate the progression of the disease and determine the appropriate course of treatment. A declining CD4 cell count indicates a worsening immune function and may necessitate adjustments in antiretroviral therapy to manage the condition effectively.

While the other options mentioned, such as the Western blot test, erythrocyte sedimentation rate (ESR), and enzyme-linked immunosorbent assay (ELISA), are relevant in the diagnosis and management of HIV/AIDS, they are not specifically used for assessing the immune status in the context of periodic monitoring.

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Is the correct order in a homeostatic feedback system stimulus, receoptor, control centre, effector?

Answers

Answer:

Correct

Explanation:

Homeostatic is nothing but ability of a system or living organism to manipulate its internal environment to maintain a stable equilibrium, for example the ability of warm-blooded animals to ascertain a constant body temperature.

The Homoeostatic feedback mechanism has three basis components and they are independent to one another.

These components are receptor, effector and integrating center. The function of receptor is to sense external stimuli and send information to integrating center. The integrating center generally hypothalmus in brain sends this signal to effector for example an organ to react to the stimuli.

So, the order  in a homeostatic feedback system stimulus, receoptor, control centre, effector is correct.

PLEASE HELP! First answer gets brainliest!!!
1. What type of plate boundaries are A, C, and D?
2. What type of crustal feature(s) will form at plate boundary D?

Answers

Answer:

transform boundaries are zones or plates a slide past each other c move together causing one to go beneath the other b move apart leaving a gap d

Which of the following terms is defined as the use of technology to change the genetic makeup of a species?

fertilization
genetic modification
genome
irrigation

Answers

Answer:

\/ \/ \/ (if this helps)

Explanation:

Genetic modification is the method of modifying an organism's genetic structure by using recombinant DNA (rDNA) technology. A gene from another animal is most commonly applied to the genome of an organism to give it a phenotype that is desired.

The answer is genetic modification. Organisms that have been changed due to genetic engineering are called GMOs. ( genetically modified organism) I hope this helps!

There are 42 gallons in one barrel of oil. How many barrels of oil are equivalent to 2,572.5 gallons of oil?

Answers

Answer:

The 62.25 barrels of oil are equivalent to 2562.5 gallons of oil

Explanation:

Given that :-

There are 42 gallons in one barrel of oil.

To find :-

How many barrels of oil are equivalent to 2,572.5 gallons of oil?

Solution :-

If 42 gallons oils in one barrel of oil

so, barrels of oil are equivalent to 2562.5 gallons of oil = 257.5/ no. of gallons in one barrel

= 257.5 / 42

barrels of oil equivalent to 2562.5 gallons of oil = 61.25

Hence, 62.25 barrels of oil are equivalent to 2562.5 gallons of oil

Which of these is a scientific hypothesis about the growth of a plant? A Plants make a pretty decoration in the house. B It's fun to watch this plant grow. C Plants grow best when they are happy. D This plant will grow best in sunlight.

Answers

Answer:

D. Plants grow best in sunlight.

Explanation:

The other answers can't be scientifically proven, therefore they would not make a good hypothesis. A hypothesis is a statement that can be tested and verified.

Plant will grow best in sunlight. So, the correct option is (D).

What is the scientific hypothesis about the growth of a plant?

According to the evolution-immunity hypothesis in ecology, fast-growing plant species should suffer more from herbivores and pathogens than slow-growing species. Tests of this hypothesis have focused on above ground plant tissues, herbivores and pathogens. This should also apply to route defense.

When the amount of fertilizer applied to the plants is increased, the growth which is the height of the plant increases. As the amount of fertilizer increases, plant growth increases.

Plant growth shall be considered as an increase in plant volume and/or mass with or without the formation of new structures such as organs, tissues, cells or cell organelles that grow in the presence of sunlight.

Thus, plant will grow best in sunlight. So, the correct option is (D).

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Photosynthesis continues to Increase with temperature.O truenot trueOtrue within limits

Answers

During photosynthesis, there ligh dependent and independent reactions. Light dependent reactions are not influenced by temperature, but light independent reactions can be affected by the increase with temperature, due to some enzyme catalysis.

The answer is: True with limits.

Part of the dermal tissue that Is made of hard, dead, cork cells is the

Answers

Answer:

Periderm!

Explanation:

Peridermis: It forms the outer bark of roots and stems of a certain age (generally greater than one year). Formed by several layers of dead cells, the most superficial form the suber or cork, with thick walls and full of air.

Imagine there was no tilt to the earths axis and earths orbit was circular

Answers

Answer:

If the Earth was not tilted on its axis, there would be no seasons. And humanity would suffer.

Explanation:

When a Mars-size object collided with Earth 4.5 billion years ago, it knocked off a chunk that would become the moon. It also tilted Earth sideways a bit, so that our planet now orbits the sun on a slant.

Hope this helps!

Brain-List?

you measure changes in membrane potential in an on-center bipolar cell that is exposed to light in the center of its receptive field. what response would you expect to see?

Answers

The response would be Depolarization due to decreased release of glutamate by the photoreceptor cell. (Option 5)

Bipolar cells can be on or off-center. Bipolar cells get inputs from a bunch of photoreceptor cells that characterize the bipolar cell's responsive field. Glutamate is the neurotransmitter released by all photoreceptor cells. A bipolar cell that responds to glutamate by excitation will be excited when the light is off because glutamate release decreases when exposed to light. Because they are active when light is off in the center of their receptive field, these cells are referred to as off-center bipolar cells.

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(complete question)

You measure changes in membrane potential in an ON-center bipolar cell that is exposed to light in the center of its receptive field. What response would you expect to see?

Select one:

1. An action potential

2. Depolarization due to increased release of glutamate by the photoreceptor cell

3. Hyperpolarization due to decreased release of glutamate by the photoreceptor cell

4. Hyperpolarization due to increased release of glutamate by the photoreceptor cell

5. Depolarization due to decreased release of glutamate by the photoreceptor cell

MULTIPLE CHOICE QUESTION
Are a majority of the problems associated with down syndrome a result
of an over or under expression of chromosome 21?
under
over

Answers

It would be over because a baby without a birth defect has 46 chromosomes but with a Down syndrome baby they have an extra copy of chromosome which is chromosome 21

Hope this helps

Have a great day/night

Does GONORRHEA What microbe causes your disease?

Answers

Gonorrhea is a sexualy transmitted deasease caused by a bacteria called Neisseria gonorrhoeae.

When it is solar noon at a location at 75º W longitude, what is the solar time at a location at 120° W longitude?

Answers

Answer:

Since solar time depends on the longitude, solar noon occurs at exactly the same moment in all locations that share your local meridian. When Is Solar Noon? In most places on Earth, solar noon does not happen at 12 o'clock. The Earth's rotation slowly shifts the meridian experiencing solar noon from east to west.

Explanation:

Using the solar time on longitude 75°W, the tone at a location which is 120°W is 9:00 a.m

Degrees of Longitude = 360°

Hours per day = 24 hours

Degrees per hour = 360° ÷ 24 = 15°

This means that for every 15° moved, 1 hour is elapsed ;

Moving 75° to 120°

Difference = 120° - 75° = 45°

Number of hours passed = (45° ÷ 15°) = 3 hours

Since the direction is Westward ; we Subtract

12:00 noon - 3:00 hrs = 9:00 a.m

Therefore, the solar time is 9:00 a.m on longitude 120°W.

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Is the plasmid-encoded fusion protein subject to regulation by camp (catabolite repression)? explain your answer.

Answers

Yes the plasmid encoded fusion protein subject to regulation by camp ( catabolite repression) because cAMP production is inhibited by glucose , in which glucose will exert catabolite repression, causing the plasmid- ended fusion protein to be subjected to regulation by cAMP.

Carbon catabolite repression ( CCR) is regulatory mechanism by which the expression of genes required for the utilization of secondary sources of carbon is prevented by the presence of preferred substrate.

Its repression was extensively studies in E.coli grows faster on glucose than on any other carbon source. E.coli is placed on agr plate containing only glucose and lactose then the bacteria will use glucose first and lactose second.

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Assign each statement to the corresponding polysaccharide. Chitin Starch Glycogen Cellulose Answer Bank is made up of two glucose polysaccharides, amylose and amylopectin provides structural support for plants is the storage form of glucose in animals provides structural support for animals such as arthropods is a storage form of fuel in plant cells consists of N-acetylglucosamine residues functions in fuel storage in animal cells Polysaccharides are polymers of monosaccharides. Homopolysaccarides are made up of only one type of monomer, whereas heteropolysaccharides are composed of at least two different kinds of monomers. Are starch, glycogen, cellulose, and chitin classified as homopolymers or heteropolymers? O All four polysaccharides are homopolymers. O All four polysaccharides are heteropolymers. O Starch is a heteropolymer, whereas chitin, cellulose, and glycogen are homopolymers. O Chitin is a heteropolymer, whereas starch, cellulose, and glycogen are homopolymers.

Answers

Chitin is a het-----eropolymer, whereas starch, cellulose, and glycogen are hom----opolymers.

What are polysaccharide?

We know that it is possible for more than one sugar unit to become joined together. The joining together of more than one sugar unit is what we call polymerization to form a polysaccharide.

There are various kinds of polysaccharides that are be formed and we are mandated to match the corrects statements that pertains to the polysaccharide;

Made up of two glucose polysaccharides, amylose and amylopectin - Cellulose

Provides structural support for plants is the storage form of glucose in animals provides structural support for animals such as arthropods - Chitin

Is a storage form of fuel in plant cells consists of N-acetylglucosamine residues functions in fuel storage in animal cells - Starch

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Part D
For each of your samples, research how the structures you observed under magnification help the
organisms carry out basic functions of life. Write your findings in the space provided.

Answers

The structures that I observed under magnification help the organisms carry out basic functions of life in a variety of ways. For example, the cell membrane helps to control what enters and leaves the cell, the nucleus contains the cell's DNA, the mitochondria produce energy, and the chloroplasts produce food for plants.

How to explain the information

The cell membrane is a thin layer that surrounds the cell and protects it from its environment. It also controls what enters and leaves the cell, allowing only certain molecules to pass through. This is important for maintaining the cell's internal environment and for carrying out essential functions such as nutrient transport and waste removal.

The nucleus is the control center of the cell. It contains the cell's DNA, which is the genetic material that determines the cell's structure and function. The nucleus also contains proteins that are involved in DNA replication, transcription, and translation.

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PLS HELP!

While five friends were playing video games, they saw a bright flash of lightening and heard a loud crash right outside the house. Being curious, they went to the window to see what had caused the loud noise. They observed a large tree branch that had been struck by lightening and fallen off a nearby tree. This sparked a little debate. Listen in on their conversation.

Alejando: Well, it looks like they tree is dead. It just lost a huge branch in the storm. Trees die when they lose big branches like that.

Quan: Nah. That tree will be fine. The branch will grow back exactly the same as it was before.

Michaela: Yeah. Haven’t you heard of cells? Cells are the basic unit of everything that is living and this tree is made up of many cells. It will be ok.

Alicia: But the tree just lost a huge branch which means it lost a lot of those cells. It can’t survive with all the cells it lost.

George: It did lose a lot of cells, but cells come from other living cells, so tree’s healthy cells should be able to produce more cells.

Which friends explanation do you agree with the most? Explain your answer.

Answers

Answer:Por otro lado, puedes raspar con un cuchillo una rama y observar si se encuentra verde. Si es así, significará que el ejemplar aún está vivo, pero si está marrón será que ha muerto. También es recomendable que muevas las ramas y compruebes su flexibilidad.

Explanation:

Answer:

You can scrape a branch with a knife and see if it is green. If so, it means that the tree is still alive, but if it is brown, it means the tree has died

Explanation:

I don't know if that helps. Hope it does though

Based on the data provided for the three stream assessment components, is the overall health and stability of the Little Gunpowder River good, fair, marginal, or poor? Explain how water quality results, physical characteristics, and the stream’s biodiversity and presence of certain macroinvertebrate species support your overall stream health rating.

Answers

Answer:

Macroinvertebrates serve several important functions within the aquatic environment:

They provide a valuable "cleaning" service by scavenging dead or decaying bacteria, plants, and animals, which helps recycle nutrients back into the system.

They are an important food for fish, birds, amphibians and reptiles.

Explanation:

the list could go on and on.

What is the function of glucagon

Answers

Glucagon counteracts the actions of insulin by by increasing blood glucose levels.

What is glucagon?

Glucagon is a peptide hormone, produced by alpha cells of the pancreas. It increases concentration of glucose and fatty acids in the bloodstream. It is considered to be the main catabolic hormone of the body. It is also used as a medication to treat a number of health conditions.

Insulin is also produced from the pancreas and it's function is to regulate the level of blood glucose in the blood stream. As alpha cell produces glucagon, beta cells produces insulin.

The main function of glucagon is to counter the effect of insulin by stimulating hepatic glucose production which will increase the blood glucose level.

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HELP i’ll brainLIST U
why are water quality standards important?

a. they help protect clean water and improve the quality of polluted water

b. industries would not self regulate and the standards force them to treat unsafe water

c. The standards guarantee that every home in the nation has safe drinking water

d. there is a limit amount of clean water in the world and like oil once it used up it’s gone

Answers

I did research on it and I choose ( a.)

11. DDT is a pesticide that can't be broken down by organisms. If a farmer sprays
DDT on the strawberry plants above, which trophic level will contain the highest
concentration of DDT?
a. Producers
b. Herbivores
c. Second-order Consumers
d. Third-order consumers

Answer quickly plz

Answers

The answer is A. I did the assignment

Compared to the endocrine system, the nervous system has afaster response, but the signal does not last as long.slower response, and a signal that does not last as long.faster response with a longer-lasting signal.slower response with a longer-lasting signal.

Answers

Answer:

Has a faster response, but the signal does not last as long.

Explanation:

The nervous system uses action potentials to transmit signals. Action potentials have a short duration and travel fast through neurons to inform in a fast way about different stimuli and allow rapid answers. On the other hand, the endocrine system uses chemical molecules to transmit their signals. As they travel through blood or together with other molecules, their signal is not transmitted as fast as in the nervous system. However, when they reach their destination and interact with the proper molecules, they start a series of changes that have a lasting effect in contrast with the nervous system.

what is an indirect measure of the amount of energy released from food?

Answers

An indirect measure of the amount of energy released from food is calorimetry. Calorimetry is a technique used to estimate the energy content of food or other substances by measuring the heat produced during its combustion or metabolism.

In a calorimetry experiment, a sample of food is burned or metabolized in a controlled environment, such as a bomb calorimeter or a metabolic chamber. The heat released during the process is measured and used to calculate the energy content of the food.

By measuring the heat energy released, calorimetry provides an indirect measure of the amount of energy present in the food. This energy content is typically expressed in units of kilocalories (kcal) or joules (J) and represents the potential energy that can be obtained from the food through metabolism.

Calorimetry is an important tool in nutrition science and is used to determine the energy value of different foods, assess energy expenditure, and evaluate the efficiency of metabolic processes. It provides valuable information for understanding the energy balance in organisms and the nutritional value of different food sources.

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How can septic tanks potentially contaminate water?
Wastewater flows into home septic tanks instead of city storage.
Septic tanks leak into coastal waters due to rising sea levels.
Using septic tanks near urban areas
Using septic tanks in rural areas

Answers

Septic tanks can contaminate water as a result of leakage into coastal water.

What are septic tanks?

Septic tanks are underground chambers created solely for the storage and treatment of domestic wastewater.

Septic tanks can be made from metal. plastic, concrete, or fiberglass, and are often used as an improvised water treatment system in rural areas. They are usually sealed and untreated wastewaters are not allowed to get into the water cycle or the environment. Pretreated wastewater is allowed to move into the drain field which consists of unsaturated soil.

The drain field further treats the water before the water eventually links up with the natural underground water.

Untreated wastewaters from septic tanks have the potential to contaminate water if they can find their way into the normal water cycle prior to treatment. For example, if there is a leakage into the nearby river or water body.

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Which of these organic molecules function to help speed up biological reactions?

Answers

Answer:

c

Explanation:

write the correct term for this definition: fluid filled chamber that has relatively few blood vessels and cushions the embryo.

Answers

The correct term for this definition is amniotic sac. The amniotic sac is a fluid-filled chamber that surrounds and cushions the developing embryo or fetus during pregnancy. It is formed from the amniotic membrane, which starts to form around the embryo soon after implantation.

The amniotic sac serves several important functions, including protecting the developing fetus from physical shock, regulating the temperature and humidity of the environment, and providing a medium for the fetus to move and develop. It also contains amniotic fluid, which is composed of water and various dissolved nutrients and waste products, and helps to cushion the fetus from external pressures and impacts.

While the amniotic sac is relatively avascular (meaning it has few blood vessels), it is supplied with nutrients and oxygen by the placenta, a specialized organ that develops alongside the embryo and provides a means of exchange between the maternal and fetal circulations.

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whats the order from biggest to smallest ecosystem,population,community,organism

Answers

Answer:

Ecosystem, community, population, Organism

Explanation:

Organism is a single species

Population is a group of the same species

Community is a group of different populations

Ecosystem is where communities live

I hope this helps...

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how does direct sunlight affect an area

Answers

direct sunlight can affect the process of photosynthesis which means photosynthesis would not be carried out well
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Simon borrows $7,000 from the bank and wants to repay the amount in equal installments of $950. Payments will be made at the end of each year. The bank wishes to earn interest on this loan at 6%. Approximately how many years will it take Simon to repay the loan Use absolute value to express the distance between 10 and 16 on the number line. What is the relationship between the law of supply and the quantity supplied?. A force of 100 N to the east and a force or 100 N to the west both act on an object of 10 kg. What is the acceleration of the object?A. 10 m/s2 to the eastB. 100 m/s2 to the westC. 0 m/s2, it does not accelerateD. 100 m/s2 to the east ____ is a gatekeeper ability that allows us to become more mindful of our communication and style and to engage in more constructive and open creation of new mental categories. Select the correct answer.Which value of x makes the equation true? Because we all springfrom different treesdoes not meanwe are not created equallyHow does this help develop a theme?Someone please help! How can expenses on groceries, clothes, and transportation be classified?A.variable expensesOB.fixed expensesC.unexpected expensesD.debt expensesResetNext the question is in the picture can someone PLSSS answer!!!i will mark brainliest!! Effective ___ is critical In order to manage projects in parts of the world where security is an environment factor affecting project success Suppose a tank leaks toxic chemicals. The area affected is a circle centered on the tank. When the spill is first noticed, the circle has a radius of 60 meters. A response team arrives 70 minutes later, by which time the affected area has grown to a circle with a radius of 80 meters.Required:How rapidly was the radius of the spill changing between the time the spill was noticed and the arrival of the response team? Riley has set up a six week payment schedule to pay for his upcoming school camp. he has already put down a 15 percent deposit and will pay the balance in 6 weekly instalments. If he paid $34.20 deposit, how much is he paying each week? Im to lazy to do this right now anyone can give me an answer Help please!!!Determine the domain and range of each function.31.32. Write 7 1/2 as a decimal number. Ohms are a measure of resistance, amperes are a measure of current, and volts are a measure of voltage. The relationship among these three quantities is represented by the given formula. Volts Ohms Amperes A 150-ohm load is connected to a 300-volt source. If the source is changed to 250 volts, what must the resistance become to ensure that the current stays the same? A. 2 ohms B. 50 ohms C. 125 ohms D. 180 ohms E. 500 ohms Come-Clean Corporation produces a variety of cleaning compounds and solutions for both industrial and household use. While most of its products are processed independently, a few are related, such as the companys Grit 337 and its Sparkle silver polish.Grit 337 is a coarse cleaning powder with many industrial uses. It costs $1.60 a pound to make, and it has a selling price of $11.60 a pound. A small portion of the annual production of Grit 337 is retained in the factory for further processing. It is combined with several other ingredients to form a paste that is marketed as Sparkle silver polish. The silver polish sells for $6.00 per jar.This further processing requires one-fourth pound of Grit 337 per jar of silver polish. The additional direct variable costs involved in the processing of a jar of silver polish are:Other ingredients $ 0.60Direct labor 1.36Total direct cost $ 1.96 The area of a circle with radius r is given by the formula A=r2. If a circle has area 81 square inches, what is the radius (in inches) What is the most important ethical issue of using AI in abusiness? Based on what you saw in the Roman-photo video, write a brief description in French of one of the video characters. Describe his or her personality traits, physical characteristics, and career path.