Which is not a difference between the biofilm formation systems in pseudomonas compared with those in vibrio species?.

Answers

Answer 1

One of the differences between biofilm formation systems in pseudomonas compared with those in vibrio species is their use of different signaling molecules. Pseudomonas uses cyclic di-GMP, whereas vibrio species use acyl homoserine lactones.

However, this is not a difference between the two biofilm formation systems. Biofilm formation is a type of bacterial growth that involves the formation of a community of bacteria. It is a complex process that is regulated by several factors such as cell adhesion, cell motility, and signaling molecules. Both Pseudomonas and Vibrio species are known for their ability to form biofilms.

However, there are some differences between the biofilm formation systems in Pseudomonas compared with those in Vibrio species. One of the differences is the signaling molecules used. Pseudomonas uses cyclic di-GMP, whereas Vibrio species use acyl homoserine lactones. Additionally, Vibrio species have a type VI secretion system that is used for cell-to-cell communication during biofilm formation, whereas Pseudomonas does not have this system.

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Related Questions

Permeability is _____ . Select one: a. the ability of a solid to allow fluids to pass through b. the process by which plants release water vapor to the atmosphere c. the amount of water vapor in the air relative to the maximum amount of water vapor the air can hold. d. the percentage of pore space in the rock

Answers

Answer:

A. The ability of a solid to allow fluids to pass through

Explanation:

Greetings !

Permeability of cell membrane refers to the ease with which a molecule can pass through a cell membrane. It is the rate at which the passive diffusion occurs through the membrane.

A solid's permeability refers to its capacity to permit the passage of fluids. As a result, option A is the right response.

A substance's permeability, such as that of rocks, is determined by how easily liquids can pass through it. Fluids can move through the porous material's network of interconnected spaces because of this property.

It is the speed at which the membrane's passive diffusion takes place. It is vital to the movement of fluids through porous media, such as the movement of groundwater in aquifers or of oil through rock formations.

As a result, permeability is the quality of a material that allows a fluid to pass through it. Option A offers the right response.

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The administration of prepared antibodies, such as those in breast milk, is an example of _____ immunity.

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The administration of prepared antibodies, such as those in breast milk, is an example of passive immunity. What is passive immunity Passive immunity is a type of immunity that occurs naturally or artificially.

In this type of immunity, an individual receives antibodies created from an external source, which grants immediate immunity against an infection. The body does not make its own antibodies in response to an infection. Passive immunity is frequently temporary, and it provides rapid defense in situations where immunity cannot wait to develop.

How can passive immunity be acquired Passive immunity can be acquired in one of two ways, as mentioned below: Artificially Acquired passive immunity is obtained through an external source, such as vaccinations. For example, when you are given an injection of immunoglobulin against hepatitis A after being exposed to the virus.NaturallyNaturally, passive immunity is acquired through vertical transmission, i.e., from mother to infant, and it provides immediate protection to the infant from some pathogens. For example, the administration of prepared antibodies, such as those in breast milk, is an example of passive immunity. This type of immunity occurs naturally in response to the transmission of maternal antibodies to the fetus across the placenta during pregnancy, as well as from the mother to the baby via breast milk. Hence, the main answer is passive immunity.

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Evaluate the model.select that do NOT accurately identify the structures labeled in the picture. Choose all that apply.

Evaluate the model.select that do NOT accurately identify the structures labeled in the picture. Choose

Answers

Answer:

C,)

D,)

Explanation:

what occurs if a cell is damaged beyond repair?

Answers

Answer:

Apoptosis

Explanation:

Answer:

Apoptosis is programmed cell death, and it usually occurs when the DNA of the cell is damaged beyond repair. Photosynthesis and glycolysis are normal metabolic processes of the cell, and would not result from irreversible damage.

Explanation:

hope this helps

the four hallmark chordate characteristics are a dorsal, hollow nerve cord, a notochord, pharyngeal gill slits and muscular segments called myomeres.

Answers

A notochord; a dorsal, hollow nerve cord, pharyngeal slits or clefts; and a muscular, post tail are the four key characteristics of chordates.

Vertebrates, tunicates, and lancelets are all part of the animal group known as the Chordates (Chordata). The vertebrates, which include fish, amphibians, birds, mammals, amphibians, and lampreys, are the most well-known of these and comprise the majority of the animal kingdom.

Chordates have a line of symmetry that separates their bodies into two roughly mirror-image halves, making them bilaterally symmetrical. The symmetry on both sides is not exclusive to chordates. Arthropods, segmented worms, and echinoderms are three more animal groups that demonstrate bilateral symmetry.

Every chordate has a notochord that is active for part of or the entirety of its life cycle.

A notochord is a semi-flexible rod that acts as an anchor for the animal's powerful body muscles and offers structural support. A core of semi-fluid cells encased in a fibrous sheath makes up the notochord. The animal's notochord runs the entire length of its body. The notochord only appears in vertebrates during the embryonic stage of development, after which it is replaced by the vertebrae that grow around it to form the backbone. The notochord is present throughout the whole life cycle of tunicates.

The complete question is:

What are the four key characteristics of chordates?

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If these examples are density independent or dependent limiting factor

If these examples are density independent or dependent limiting factor

Answers

In ecology, density is a measure that refers to how many individuals a population has in a specific habitat.

Considering this, we can say that water is a density-dependent limiting factor, since this is a limited resource that can vary its availability annually, but also is a species have too many individuals, all of them will not have the same capacity to reach this resource, especially when we talk about large animals or plants.

Diseases caused by bacteria would be also a density-dependent limiting factor, since only when too many microorganisms of a pathogenic species replicate in our bodies, do we begin to feel the symptoms of that illness.

Predators are also a density-dependent limiting factor since the species which they feed on can be overhunted by them, being reduced in number of individuals, or being not too much hunted and increase their individuals.

Earthquakes are a density-independent limiting factor, since they do not depend on other factors to occur, but only on the movement of our planet's plates.

Finally, limits population regardless of how many organisms are present in the area is a density-independent limiting factor, since they do not depend on how many organisms they're to limit the increase of the population. Being this option an abiotic kind of non-limiting factor.

Need help quiz is here and this is the only answer I don’t know

Need help quiz is here and this is the only answer I dont know

Answers

Answer:

activation energy is the answer

GIZMOS NITROGEN CYCLE SCIENCE LAB!

Which arrows do I click? (One for each row)

GIZMOS NITROGEN CYCLE SCIENCE LAB!Which arrows do I click? (One for each row)

Answers

Explanation:

Ammonia is down, Nitrites is up, Nitrates is side to side

Answer:

Explanation:

Ammonia side to side

Nitrates NO2 down

Nitrates NO3 Up

Explain how shape is important in insulin's ability to send messages to
cells about blood glucose levels.

Answers

The shape of the receptor is essential in insulin's ability to send messages to cells about blood glucose levels and glucose out of your bloodstream and carry it into cells throughout your body. The cells then utilise the glucose for energy and keep the extra glucose in liver, muscles, and fat tissue.

Which part of this negative feedback loop is the stimulus  ?

A negative feedback loop takes place in the field of biology specifically in processes of homeostasis, when the product of the reaction leads to a decrease in the overall process.

Through this, the stimulus of a negative feedback loop brings a system to bring system to equilibrium or stability.

In this provided case increase in glucose level in the blood is acts as the stimulus to release the insulin hormone that return the blood glucose level back to normal.

Thus, his could be the answer.

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21.As mentioned in the video, the environment can also affect an organism's traits. The example of nourishment was used in the case for Spike, as this could affect his growth and size. This can also occur in humans. UV light was not mentioned in the video. How could UV light potentially affect an organism's trait? Provide one example.

Answers

Answer:

UV light can modify the DNA of a human being, provoking mutations that affect the traits. For example, exposure to the sun's UV rays affects the DNA of the skin cells, causing mutations that can lead to cancer.

Explanation:

An environmental factor that can affect humans traits is the exposure to UV light that the sun radiates. The DNA in our skin cells absorbs the UV rays. As a result, the DNA can suffer modifications that result in skin cancer. Also, the absorption of UV rays causes wrinkles since cell components are affected due to the accumulation of UV light.

Answer: UV light can modify the DNA of a human being, provoking mutations that affect the traits. For example, exposure to the sun's UV rays affects the DNA of the skin cells, causing mutations that can lead to cancer.

Explanation: An environmental factor that can affect humans traits is the exposure to UV light that the sun radiates. The DNA in our skin cells absorbs the UV rays. As a result, the DNA can suffer modifications that result in skin cancer. Also, the absorption of UV rays causes wrinkles since cell components are affected due to the accumulation of UV light.

Rabbits were introduced to Australia in the 1800s and spread quickly across
the continent, moving into the nests of native burrowing animals and eating
their food. What allowed the nonnative rabbits to spread so quickly in
Australia?
A. The rabbits had no tolerance for the niches in Australia.
B. The rabbits were able to live in niches that Australian animals
could not live in.
C. The rabbits had a high tolerance for the abiotic factors in
Australia.
D. The rabbits were smarter than the Australian predators.

The answer is C ( ◠‿◠ )

Answers

The answer you are looking for is C

g. mu~noz-gilet al., objective comparison of methods to decode anomalous diffusion.nat.commun.12, 6253 (2021)

Answers

The article titled "Objective comparison of methods to decode anomalous diffusion" was published by G. Muñoz-Gil et al. in Nature Communications on 2021.

The article explores different methods used to analyze anomalous diffusion, which refers to the movement of particles or molecules that deviates from classical diffusion patterns.

Anomalous diffusion is commonly observed in various biological and physical systems. The authors aim to provide an objective evaluation of the existing methods for characterizing and quantifying anomalous diffusion.

The study compares different algorithms and approaches using simulated and experimental data to assess their accuracy and reliability. The findings contribute to the development of improved methods for understanding and analyzing anomalous diffusion phenomena.

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Explain the process of photosynthesis using the picture

Explain the process of photosynthesis using the picture

Answers

Answer: the sun provides energy to the plant. And the plant turns the solar energy into chemical energy through photosynthesis. this provides the plant with energy

Explanation:

Which process provides plants with the glucose needed for cellular respiration?.

Answers

Answer:

Photosynthesis is the process that plants use in the presence of sunlight to convert carbon dioxide taken in by their leaves and water taken in through the roots to produce oxygen and sugar (glucose).

How the oxygen levels have an effect on rivers and streams?

Answers

The amount/type of fish or plant in the water

Answer:

All terrestrial and aquatic animals need oxygen to survive. Many aquatic macroinvertebrate species depend on oxygen-rich water. Without sufficient oxygen they may disappear. Even a small change in dissolved oxygen concentration can affect the composition of aquatic communities. Many fish require a certain dissolved oxygen range in order to survive

Aquatic life- animals living in water use up dissolved oxygen. Bacteria take up oxygen as they decompose materials. Dissolved oxygen levels drop in a water body that contains a lot of dead, decomposing material.

Elevation- the amount of oxygen in elevation increases. Since streams get much of their oxygen from the atmosphere, streams at higher elevations will generally have less oxygen.

Salinity (saltiness)- Salty water holds less oxygen than fresh water.

Temperature- cold water holds more dissolved oxygen than warm water.

Turbulence- more turbulence creates more opportunities for oxygen to enter streams.

Vegetation- riparian vegetation directly affects dissolved oxygen by releasing oxygen into the water during photosynthesis. It indirectly affects dissolved oxygen concentrations because vegetation shading a stream may decrease water temperatures, and as temperature decreases dissolved, oxygen increases.

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Zones of blood flow through the lungs are most directly determined by which of the following?

a. the person's arterial blood pressure
b. the height of a person
c. the septa dividing regions fed by specific bronchi
d. the relationship between alveolar pressure and capillary pressure

Answers

The relationship between alveolar pressure and capillary pressure. The correct answer is d.

The zones of blood flow through the lungs are primarily determined by the relationship between alveolar pressure and capillary pressure. This concept is known as the zone model of pulmonary circulation.In the zone model, the lung is divided into three zones based on the relative pressures in the alveoli and pulmonary capillaries:Zone 1: This zone occurs in the apex of the lung and is characterized by the lowest blood flow.Zone 2: This zone occurs in the middle region of the lung. Here, pulmonary capillary pressure exceeds alveolar pressure during systole but drops below alveolar pressure during diastole.Zone 3: This zone occurs in the base of the lung, which has the highest blood flow. In this zone, alveolar pressure is lower than pulmonary capillary pressure throughout the cardiac cycle, resulting in continuous blood flow.

Factors such as a person's arterial blood pressure (a) or height (b) do not directly determine the zones of blood flow in the lungs. Similarly, the septa dividing regions fed by specific bronchi (c) are not directly involved in determining the zones of blood flow. The correct answer is d.

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Decomposers chemically break
down waste and leftovers. What
group is responsible for
mechanically breaking down the
leftovers?
A. detritivores
B. primary consumers
C. carnivores
D. producers

Answers

The group that is responsible for mechanically breaking down the

leftovers are detritivores. They are also called decomposers.

Detritivores and descomposers

Decomposers are heterotrophic organisms that obtain their food by breaking down organic substances.

Moreover, detritivores are a special type of decomposer (heterotrophic organisms) that obtains their food by consuming decomposed parts of plants and animals.

Detritivores are decomposers that are also known to consume different types of animal feces.

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Answer: A. detritivores

Explanation:

What happens during G₂ phase?
O A. DNA separates into two nuclei.
O B. Chromosomes are duplicated.
OC. Chromosomes are checked for errors.
D. Organelles are manufactured.

Answers

During the G₂ phase, the Organelles are manufactured. That is option D.

What is cell cycle?

The cell cycle is defined as the systematic events that occurs in a cell as it grows and multiplies. There are various stages of cell cycle and they include the following:

synthesis phase (S)Gap one phase (G1)Gap 2 phase (G2)Mitosis phase (M)

The events that takes place at the gap 2 phase of the cell cycle include the following:

cell produces additional organelles andproteins necessary for cell division.

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the lymphatic vessel that collects lymph from the left chest, abdomen, and lower limbs is called the:

Answers

The lymphatic vessel that collects lymph from the left chest, abdomen, and lower limbs is called the thoracic duct.

The thoracic duct is the largest lymphatic vessel in the body and plays a crucial role in the lymphatic system. The thoracic duct begins in the abdomen, near the second lumbar vertebra, where it collects lymph from the intestinal lymphatic vessels called lacteals. It ascends through the thoracic cavity, alongside the aorta, and collects lymph from various organs and tissues along its course, including the left chest, left arm, left side of the head, neck, and upper thorax. Eventually, the thoracic duct drains into the left subclavian vein near the junction of the left internal jugular vein, returning the lymph back into the bloodstream. This junction is an important site where lymph from the thoracic duct combines with venous blood, allowing the transfer of immune cells and waste products from the lymphatic system to the circulatory system. The thoracic duct serves as a major pathway for the return of lymph and plays a crucial role in maintaining fluid balance, immune response, and nutrient absorption within the body.

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The lymphatic vessel that collects lymph from the left chest, abdomen, and lower limbs is known as the thoracic duct. The thoracic duct is a significant lymphatic vessel in the body that collects lymph from the lower limbs, abdominal, and left side of the chest, and empties it into the bloodstream.

The lymphatic system is a network of vessels and tissues that are part of the immune system, responsible for transporting lymph fluids throughout the body. The thoracic duct is the largest lymphatic vessel in the body, about 40-45cm long, and starts from the level of T12 and ascends through the diaphragm into the chest cavity.

The duct runs parallel to the vertebral column and carries lymph fluid through the thorax and abdomen, draining into the junction of the left subclavian and internal jugular veins. The thoracic duct is responsible for returning excess fluid and protein to the bloodstream and absorbing fats and fat-soluble vitamins from the digestive system.

In conclusion, the thoracic duct is the primary lymphatic vessel that collects lymph from the left chest, abdomen, and lower limbs. It is responsible for returning excess fluid and protein to the bloodstream and plays an important role in maintaining fluid balance in the body.

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The sides of the dna molecule are made up of repeating nitrogen bases and sugars.

Answers

Answer:

BRAINLY

ME p     l    z

Explanation:

T or F - The sides of the DNA molecule are made up of repeating nitrogen bases and sugars. T or F - The letters that make up the DNA molecule code for genes. T or F - Replication results in two strands of DNA, each of which has half of the original strand.

Please help me, I don't understand this

Please help me, I don't understand this

Answers

Answer:

4, based on the assumption that the R allele is dominant over the r allele, and that the T allele is dominant over the t allele.

Explanation:

Any bush with a dominant petal color allele (R) will have red petals. This includes Rr and RR.

Any bush with a dominant bush size allele (T) will have a tall bush. This includes Tt and TT.

The only way to acquire a short bush or pink petals is to have two recessive alleles together (tt, rr).

RRTT, RrTT, RRTt, and RrTt -> red petals with tall bushes

RRtt, Rrtt -> Red petals with short bushes

rrTt, rrTT -> pink petals and tall bushes

rrtt-> pink petals with short bushes.

These are the four phenotype variations possible in this dihybrid cross.

Select the correct answer.
A gene with the sequence ATTCATTCA underwent duplication after a few generations. Which sequence represents this gene after the mutation?
Group of answer choices

ATCATTCA

ACTTACTTA

ATTCACATTCA

ATCCATTCA

ATTCATTCA

Answers

Answer: ATCATTCA

Explanation:

What's question 1-3??

What's question 1-3??

Answers

Answer:

Farmers, Ranchers, Favorable, Generations, Mate, Inherit. Hope this helps

Explanation:

Answer:

1. Farmers, ranchers... What they're doing is called artificial selection.

2. favorable, generation

3. mate, inherit

Which of the following is a correct sequence of events in cellular respiration?

Answers

The correct sequence of events in cellular respiration is option a: glycolysis, citric acid cycle, electron transport chain.

During cellular respiration, glucose is broken down to produce energy in the form of ATP. The process starts with glycolysis, which occurs in the cytoplasm and involves the breakdown of glucose into pyruvate molecules. Glycolysis generates a small amount of ATP and NADH.

The pyruvate molecules produced in glycolysis enter the mitochondria, where the citric acid cycle, also known as the Krebs cycle, takes place. In this cycle, the pyruvate is further broken down, releasing carbon dioxide and generating NADH and FADH2 as electron carriers. The citric acid cycle also produces a small amount of ATP.

The electron carriers NADH and FADH2 then participate in the electron transport chain, which is located in the inner membrane of the mitochondria. In the electron transport chain, the electrons from NADH and FADH2 are transferred through a series of protein complexes, creating a flow of electrons that drives the synthesis of ATP. This process is known as oxidative phosphorylation.

Therefore, the correct sequence of events in cellular respiration is glycolysis, citric acid cycle, and electron transport chain, as stated in option a.

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Question

Which of the following is the correct sequence of events in cellular respiration?

a. glycolysis, citric acid cycle, electron transport chain

b. glycolysis, preparatory reaction, citric acid cycle, electron transport chain

c. glycolysis, electron transport chain, preparatory reaction

d. citric acid cycle, glycolysis, electron transport chain, preparatory reaction

e. citric acid cycle, electron transport, glycolysis, preparatory reaction

differentiate nebular hypothesis and solor nebula theory​

Answers

Explanation:

The Nebular hypothesis is the most widely accepted model in the field of cosmogony to explain the formation and evolution of the Solar System (as well as other planetary systems). It suggests that the Solar System formed from gas and dust orbiting the Sun. The theory was developed by Immanuel Kant and published in his Allgemeine Naturgeschichte und Theorie des Himmels ("Universal Natural History and Theory of the Heavens"), published in 1755 and then modified in 1796 by Pierre Laplace.

The solar nebular hypothesis describes the formation of our solar system from a nebula cloud made from a collection of dust and gas. It is believed that the sun, planets, moons, and asteroids were formed around the same time around 4.5 billion years ago from a nebula.

The law of what
states that trans are passed from parents to
offspring independently of one another.

Answers

Answer:

genetic inherent

Explanation:

I ahve no clue

law of independent segregation(Gregor Mendel) i think

Mouse coat color (white or brown) is determined by genes in the main genome. If a somatic nucleus from a white mouse skin cell was inserted into a brown mouse egg cell (with its nucleus removed), what color would you expect in the cloned mouse?

Answers

The mouse will have the nuclear genetic material of the white mouse's somatic cell, therefore it's expected for the cloned mouse to be white.

What do you call the union of egg and sperm?
menstruation
circumsion
ovulation
fertilization

Answers

Answer:

Fertilization

Explanation:

Fertilization is the joining of gametes (egg and sperm) to form a zygote.

Fertilization is the union of egg and sperm also called a zygote which is a fertilized egg cell that results from the union of a female gamete

Whole Blood Gene Expression Profiles to Assess Pathogenesis and Disease Severity in Infants with Respiratory Syncytial Virus Infection

Answers

Whole blood gene expression profiles can be used to assess the pathogenesis and disease severity in infants with respiratory syncytial virus (RSV) infection.

Whole blood gene expression profiling involves analyzing the activity of thousands of genes in the blood sample of an individual. In the case of infants with RSV infection, this technique can provide valuable insights into the underlying molecular mechanisms involved in the pathogenesis of the infection and the severity of the disease.

By comparing the gene expression profiles of infected infants to healthy controls, researchers can identify specific genes and pathways that are dysregulated during RSV infection. This information can help in understanding the immune response to the virus, identifying potential biomarkers for disease severity, and discovering novel therapeutic targets.

Additionally, whole blood gene expression profiling allows for non-invasive sampling, making it suitable for studying infants who are more susceptible to severe RSV infections. Overall, this approach holds promise for improving our understanding of RSV pathogenesis and developing targeted interventions for affected infants.

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A burn caused by boiling water causes proteins inside your cells to lose their functions. Explain why the high temperature causes this to happen.A. The increased temperature disrupts covalent bonds such as peptide bonds which causes the proteins to lose their primary structures and abilities to catalyze reactions.B. The increased temperature disrupts weak interactions such as hydrogen bonding which causes the proteins to lose their primary structures and abilities to catalyze reactions.C. The increased temperature disrupts weak interactions such as hydrogen bonding which causes the proteins to lose their tertiary structures and abilities to bind specific molecules.D. The increased temperature disrupts covalent bonds such as peptide bonds which causes the proteins to lose their tertiary structures and abilities to bind specific molecules.

Answers

Option B. The increased temperature disrupts weak interactions such as hydrogen bonding which causes the proteins to lose their tertiary structures and abilities to bind specific molecules.

The increased temperature disrupts weak interactions such as hydrogen bonding which causes the proteins to lose their tertiary structures and abilities to bind specific molecules.

High temperature can cause proteins to denature, or lose their three-dimensional structure, due to the disruption of weak interactions such as hydrogen bonding, van der Waals forces, and hydrophobic interactions. This disruption can cause the protein to lose its ability to bind to specific molecules, which can affect its function. Covalent bonds, such as peptide bonds, are stronger and require even higher temperatures to be disrupted. The loss of protein function can lead to a range of cellular and physiological effects, including tissue damage and cell death, as seen in burns caused by boiling water.

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need help with this question:A classmate asks, generally, when it is ok to use OLS estimatesand when you need to worry about identification. What can you tellthem? you have a reservoir held at a constant temperature of 30c. you add 400 j of heat to the reservoir. if you have another reservoir at 0c, how much heat must you add to it so that both reservoirs have the same increase in entropy? (1 point) Write complete sentences, saying what the following people like to do. MODELO: Selena / contestar en clase A Selena le gusta contestar en clase. 10. mi primo Ernesto / la comida de Puerto Rico 11. nosotros / las playas de Puerto Rico 12. calculate srxn for the balanced chemical equation: 2h2s(g) 3o2(g)2h2o(g) 2so2(g) Dr. Green decides to examine the relationship between parental perception of crying (how upset they think the infant is) and the parent's emotional regulation. He recruits parents to participate in his survey online, which consists of listening to cry sounds (of other infants, not their own) and rating how upset they think the infant is. Then he has them complete a survey on to measure their general emotional regulation. He would like to do a follow up survey, so he collects names and email addresses for each parent participant. Parents are hearing cry sounds which is what they are used to hearing; and because these cry sounds are not from their own children, they are expected to not add to the parents' stress levels. There is no societal risk for their names to be associated with the data (but data will be held confidential). This research would be categorized as research. expedited not eligible for human subjects research guidelines exempt greater than minimal risk full board which of the following geographic concepts can be identified using information evident in the image? responses cultural diffusion cultural diffusion acculturation acculturation forced migration forced migration sequent occupance sequent occupance cultural relativism A student's tuition was $3000. A loan was obtained for 5/6 of the tuition. How much was the loan? WILL ARK BRAINLY! Evaluate.54^0 p(rolling an 8) with one regular die if you roll the die just once ASAP PLASE GIVING BRAINLEST Select the correct answer.Each statement describes a transformation of the graph of /(4) = ?2. Which statement correctly describes the graph of y= *x-7) + 37 What is the standard deviation of 7? Camille received notice from the tax assessor's office that her home's assessed value is $100,000. The notice also indicates an assessment rate of 35%. 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